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Profiling of ADAM12S regulated proteins in H1688 cells

ABSTRACT: In this project, we used SILAC quantitative proteomics to identify proteins that are differentially regulated by the short secreted form of A Disintegrin And Metalloproteinase 12, ADAM12S, in a small cell lung cancer cell line H1688. The aim of this study is to discover the proteins that are responsible for the cell proliferation, migration, and invasion and then to elucidate the molecular mechanism by which ADAM12S promotes these processes in small cell lung cancer cells.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

DISEASE(S): Lung Small Cell Carcinoma

SUBMITTER: Dan Li

LAB HEAD: Guoqiang Xu

PROVIDER: PXD013242 | Pride | 2021-02-15

REPOSITORIES: Pride

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			Action	DRS
	SILACBiologicalreplicate1-Fraction1.raw	Raw
	SILACBiologicalreplicate1-Fraction10.raw	Raw
	SILACBiologicalreplicate1-Fraction11.raw	Raw
	SILACBiologicalreplicate1-Fraction12.raw	Raw
	SILACBiologicalreplicate1-Fraction13.raw	Raw

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Publications

SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by Which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1.

Duan Qianqian Q Li Dan D Xiong Lipeng L Chang Zenghui Z Xu Guoqiang G

Journal of proteome research 20190607 7

Small cell lung cancer (SCLC) accounts for ∼14% of total lung cancer, which is the worldwide leading cause of morbidity and mortality in cancer. Although SCLC can be treated with chemotherapy and radiotherapy, its 5 year survival rate is still below 7%. Therefore, it is essential to discover new molecules and elucidate the underlying mechanisms modulating the tumorigenesis and metastasis of SCLC for the unmet medical needs. The secreted form of A Disintegrin And Metalloproteinase 12 (ADAM12S) is ...[more]

PMID: 31117637

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