Dataset Information

Label free mass spectrometry quantification of Akt interacting proteins in insulin stimulated cardiomyocytes from Metabolic Syndrome Rats.

ABSTRACT: In the present work, we used a label-free mass spectrometry approach to identify changes in the abundance of Akt-interacting proteins in cardiomyocytes of MetS rats. Using this strategy, we were able to identify specific proteins with evident dissimilarities in their abundance within the Akt complex. Our data provide for the first time proteomic support to the documented impairment in energy metabolism and insulin signaling in the heart as a consequence of the MetS condition.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart, Cardiac Muscle Cell

DISEASE(S): Abdominal Obesity-metabolic Syndrome

SUBMITTER: Jesus Alberto Olivares

LAB HEAD: Jesús A. Olivares Reyes

PROVIDER: PXD013260 | Pride | 2020-02-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180517_HDMSE_C_001.raw.zip	Raw
	20180517_HDMSE_SM_001.raw.zip	Raw
	20180517_MSE_C_001.raw.zip	Raw
	20180517_MSE_SM_001.raw.zip	Raw
	20180517_UDMSE_C_001.raw.zip	Raw

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Publications

Metabolic syndrome diminishes insulin-induced Akt activation and causes a redistribution of Akt-interacting proteins in cardiomyocytes.

Landa-Galvan Huguet V HV Rios-Castro Emmanuel E Romero-Garcia Tatiana T Rueda Angelica A Olivares-Reyes Jesus Alberto JA

PloS one 20200129 1

Metabolic syndrome (MetS) is a cluster of cardiometabolic risk factors, with insulin resistance as a critical component for its development. Insulin signaling in the heart leads to Akt (also known as PKB) activation, a serine/threonine protein kinase, which regulates cardiac glucose metabolism and growth. Cardiac metabolic inflexibility, characterized by impaired insulin-induced glucose uptake and oxidation, has been reported as an early and consistent change in the heart of different models of ...[more]

PMID: 31995605

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