Proteomics

Dataset Information

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HiRIEF-LC-MS based proteomics analysis of subcellular fractions of human CD4+ T-Cells with and without T-cell Receptor stimulation.

ABSTRACT: Subcellular proteomics analysis of human T-cells in response to T-cell receptor stimulation. High Resolution Isoelectric Focusing (HiRIEF) LC-MS/MS and relative quantification by TMT 10-plex was used to analyze subcellular fractions (Cytosol, Nuclear and Mitochondria-Small Organelle-Membrane) of Human T-cells from 3 separate donors stimulated with T-cell receptor stimulation for 0, 15 minutes or 60 minutes. The data was obtained from 3 separate TMT10 plex sets which included: 126 – Cytosol, unstimulated; 127N – Cyto, 15 min; 127C – Cyto, 60 min; 128N – Mitochondrion-Small Organelle-Membrane, unstimulated; 128C – Mito-SmO-Mem, 15 min; 129N – Mito-SmO-Mem, 60 min; 129C – Nuclear, unstimulated; 130N – Nuc, 15 min; 130C– Nuc, 60 min; 131 – pooled internal control of all samples.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, T Cell, T-lymphocyte, Blood

DISEASE(S): Disease Free

SUBMITTER: Mattias Vesterlund  

LAB HEAD: Janne Lehtiö

PROVIDER: PXD013284 | Pride | 2019-11-13

REPOSITORIES: Pride

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Rubin_RJ15F3_20160901_donor1_QE_TMT10_175ugIPG3-10_7of15ul_fr01.raw Raw
Rubin_RJ15F3_20160901_donor1_QE_TMT10_175ugIPG3-10_7of15ul_fr02.raw Raw
Rubin_RJ15F3_20160901_donor1_QE_TMT10_175ugIPG3-10_7of15ul_fr03.raw Raw
Rubin_RJ15F3_20160901_donor1_QE_TMT10_175ugIPG3-10_7of15ul_fr04.raw Raw
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Publications

TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells.

Joshi Rubin Narayan RN   Stadler Charlotte C   Lehmann Robert R   Lehtiö Janne J   Tegnér Jesper J   Schmidt Angelika A   Vesterlund Mattias M  

Frontiers in immunology 20191126

We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237  ...[more]

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