Proteomics

Dataset Information

0

Human Transketolase-like 1 protein TAP-LC-MS/MS in HeLa cells.


ABSTRACT: The human genome encodes two proteins closely related to transketolase (TKT), transketolase-like protein 1 and 2 (TKTL1 and TKTL2). TKTL1 shares 61% amino acid sequence identity with TKT. More and more evidence suggests a role for TKTL1 in proliferation or cell cycle regulation. Indeed, TKTL1 is overexpressed in various cancers and is correlated with poor prognosis in colon, urothelial, gastric, and lung cancers as well as in ocular adnexa carcinomas, rectum carcinomas, and laryngeal squamous cell carcinomas. Increased TKTL1 levels also correlate with esophageal squamous cell carcinoma metastasis and increased resistance against cisplatin chemotherapy in nasopharyngeal carcinomas. Moreover, TKTL1 overexpression promotes cell proliferation and enhanced tumor growth; in contrast, TKTL1 down regulation attenuates the proliferation of various types of cancer cells. However, the cellular functions and regulatory machineries of TKTL1 are still largely unknown. Through this proteomic analysis, we defined the protein-protein interaction map for the TKTL1 and uncovered the novel regulating function of TKTL1 in regulating TKT transketolase activity.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Yang Li  

LAB HEAD: Jianyuan Zhao

PROVIDER: PXD013309 | Pride | 2019-11-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F002609.dat Other
TKT.RAW Raw
TKT.mgf Mgf
TKTL1.RAW Raw
TKTL1.mgf Mgf
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Publications

APC/C<sup>CDH1</sup> synchronizes ribose-5-phosphate levels and DNA synthesis to cell cycle progression.

Li Yang Y   Yao Cui-Fang CF   Xu Fu-Jiang FJ   Qu Yuan-Yuan YY   Li Jia-Tao JT   Lin Yan Y   Cao Zhong-Lian ZL   Lin Peng-Cheng PC   Xu Wei W   Zhao Shi-Min SM   Zhao Jian-Yuan JY  

Nature communications 20190607 1


Accumulation of nucleotide building blocks prior to and during S phase facilitates DNA duplication. Herein, we find that the anaphase-promoting complex/cyclosome (APC/C) synchronizes ribose-5-phosphate levels and DNA synthesis during the cell cycle. In late G<sub>1</sub> and S phases, transketolase-like 1 (TKTL1) is overexpressed and forms stable TKTL1-transketolase heterodimers that accumulate ribose-5-phosphate. This accumulation occurs by asymmetric production of ribose-5-phosphate from the n  ...[more]

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