Dataset Information

Impact of increased core fucosylation on the exosome proteome in prostate cancer

ABSTRACT: Prostate cancer is the most commonly diagnosed oncogenic malignancy in men worldwide, resulting in almost 30,000 cancer-related deaths in the United States each year. Wider examination of aberrant glycosylation in prostate cancer has revealed increased expression of the glycotransferase involved in core fucosylation, (1,6)fucosyltranferase (FUT8) associating with aggressive (AG) prostate cancer and castration-resistance, with functional analyses revealing FUT8 impacting cell motility and invasiveness in prostate cancer cells. Exosomes are extracellular microvesicles (30-150 nm) that are involved in in both proximal and distal intercellular communication via the transport of proteins and nucleic acids (mRNA, miRNA, and DNA). To gain insight into the impact of increased cellular FUT8 expression on exosome biogenesis and protein cargo profiles in prostate cancer, we paired Nanoparticle Tracking Analysis (NTA) and stable isotope labelling with amino acids in cell culture (SILAC) quantitative proteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Prostate, Prostate Cancer Cell, Cell Culture

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: David Clark

LAB HEAD: Hui Zhang

PROVIDER: PXD013340 | Pride | 2020-05-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DC_LAPC4-EXO_SILAC_Global-F10_170828_Rep01.mzML	Mzml
	DC_LAPC4-EXO_SILAC_Global-F10_170828_Rep01.raw	Raw
	DC_LAPC4-EXO_SILAC_Global-F10_170828_Rep02.mzML	Mzml
	DC_LAPC4-EXO_SILAC_Global-F10_170828_Rep02.raw	Raw
	DC_LAPC4-EXO_SILAC_Global-F10_170828_Rep03.mzML	Mzml

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Publications

Impact of Increased FUT8 Expression on the Extracellular Vesicle Proteome in Prostate Cancer Cells.

Clark David J DJ Schnaubelt Michael M Hoti Naseruddin N Hu Yingwei Y Zhou Yangying Y Gooya Mahta M Zhang Hui H

Journal of proteome research 20200518 6

Extracellular vesicles (EVs) are involved in intercellular communication, transporting proteins and nucleic acids to proximal and distal regions. There is evidence of glycosylation influencing protein routing into EVs; however, the impact of aberrant cellular glycotransferase expression on EV protein profiles has yet to be evaluated. In this study, we paired extracellular vesicle characterization and quantitative proteomics to determine the systemic impact of altered α(1,6)fucosyltranferase (FUT ...[more]

PMID: 32378902

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