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Identification of human proteins whose binding to the N6-methyladenosine-containing hairpin from 28S rRNA is modulated by methylation

ABSTRACT: We have recently found that the human methyltransferase ZCCHC4 is responsible for introducing the single m6A modification in 28S rRNA. The project is aimed at further elucidating the functional consequences of such methylation. To this end, protein mass spectrometry is utilized to identify proteins that bind preferentially to the methylated or unmethylated form of the m6A containing hairpin sequence in 28S rRNA. Specifically, this is done by incubating a HeLa cell extract with an methylated or unmethylated RNA oligonucleotide containing a biotin affinity tag, which is used to pull-down the RNA with associated proteins.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervix Epithelium, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Pascal Jansen

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD013354 | Pride | 2019-11-22

REPOSITORIES: Pride

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			Action	DRS
	20181025_Fusion1_SA_MaB_CG_RNAPal_F.raw	Raw
	20181025_Fusion1_SA_MaB_CG_RNAPal_R.raw	Raw
	txt.zip	Other

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