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Identification of proteins synthesized from long-lived mRNAs during seed germination in rice

ABSTRACT: To identify the proteins synthesized from long-lived mRNAs at early phase of germination, proteome of embryos at 0 DAI (dry embryos), 1 DAI with α-amanitin and 1 DAI without α-amanitin were analyzed by using LC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Embryo

SUBMITTER: Hirofumi Nakagami

LAB HEAD: Hirofumi Nakagami

PROVIDER: PXD013628 | Pride | 2019-08-13

REPOSITORIES: Pride

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Publications

Shotgun Proteomic Analysis Highlights the Roles of Long-Lived mRNAs and De Novo Transcribed mRNAs in Rice Seeds upon Imbibition.

Sano Naoto N Takebayashi Yumiko Y To Alexandra A Mhiri Corinne C Rajjou Loï C LC Nakagami Hirofumi H Kanekatsu Motoki M

Plant & cell physiology 20191101 11

During seed germination, proteins are translated not only from mRNAs newly transcribed upon imbibition but also from long-lived mRNAs that are synthesized during seed maturation and stored in the mature dry seeds. To clarify the distinct roles of proteins translated from long-lived mRNAs and de novo transcribed mRNAs in germinating rice embryos, proteome analysis based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combining the use of a transcriptional inhibitor was performed. We ...[more]

PMID: 31373371

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