Dataset Information

The tumor suppressor SCRIB is a negative modulator of the Wnt/-catenin signaling (part 2)

ABSTRACT: Epithelial tissues are highly organized structures that rely on cell polarity pathways driven by membrane receptors and scaffolding proteins. SCRIBBLE is a cytoplasmic scaffold present at cell-cell junctions in polarized cells that contains LRR and PDZ domains. The current interactome of SCRIB and LRRC1 consists of 67 and 30 protein interaction partners respectively with only 9 (10%) common partners (BioGRID, version 3.5). Our study identified a protein complex associated to SCRIB stabilized by the inhibition of the proteasome and further characterize SCRIB act as negative modulator of the Wnt/β-catenin signaling.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell

SUBMITTER: Luc Camoin

LAB HEAD: Camoin Luc

PROVIDER: PXD013636 | Pride | 2019-09-25

REPOSITORIES: Pride

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			Action	DRS
	Controle_DMSO.raw	Raw
	Controle_DMSO_161022182048.raw	Raw
	Controle_DMSO_161022205338.raw	Raw
	Controle_MG132.raw	Raw
	Controle_MG132_161023031844.raw	Raw

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Publications

The Tumor Suppressor SCRIB is a Negative Modulator of the Wnt/β-Catenin Signaling Pathway.

Daulat Avais M AM Wagner Mônica Silveira MS Walton Alexandra A Baudelet Emilie E Audebert Stéphane S Camoin Luc L Borg Jean-Paul JP

Proteomics 20191020 21-22

SCRIB is a scaffold protein containing leucine-rich repeats (LRR) and PSD-95/Dlg-A/ZO-1 domains (PDZ) that localizes at the basolateral membranes of polarized epithelial cells. Deregulation of its expression or localization leads to epithelial defects and tumorigenesis in part as a consequence of its repressive role on several signaling pathways including AKT, ERK, and HIPPO. In the present work, a proteomic approach is used to characterize the protein complexes associated to SCRIB and its paral ...[more]

PMID: 31513346

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