E. coli Affinity purification MS/MS
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ABSTRACT: The tRNA linked repeat sequence rrB was tagged in either 5'- or 3'- end with the MS2 aptamer and expressed from a plasmid in E. coli. Using the MS2 aptamer, the RNAs were affinity purified after cell lysis. The co-purifying proteins were identified by NanoLC-MS/MS and compared to the proteins co-purifying with the MS2 sequence alone.
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Escherichia Coli
TISSUE(S): Cell Culture
SUBMITTER: Thoams Stenum
LAB HEAD: Michael Askvad Sørensen
PROVIDER: PXD013749 | Pride | 2021-04-26
REPOSITORIES: Pride
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