Proteomics

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TFEB Immunoprecipitation And Proteome

ABSTRACT: Our studies in Salmonella Typhimurium (S. Typhimurium)-infected cells indicate that TFEB has a non-transcriptional, cytosolic function in addition to its well-characterized role as a transcription factor in regulating autophagy and lysosome biogenesis. An unbiased proteomics approach revealed that TFEB interacts with several mitochondrial proteins, which is lost when infected with S. Typhimurium. Microscopical and biochemical examinations further confirmed a novel localization of TFEB in mitochondria. We have also identified a TOMM20 binding motif within the protein sequence of TFEB which, facilitates the mitochondrial translocation in a mTOR-dependent manner. Our results further demonstrate that TFEB and the mitochondrial protease LonP1 co-regulate the assembly of complex I and its function. Moreover, during S. Typhimurium infection, lack of TFEB specifically in the mitochondria exacerbates the expression of pro-inflammatory cytokines.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Hendrik Nolte  

LAB HEAD: Marcus Krüger

PROVIDER: PXD013758 | Pride | 2024-05-21

REPOSITORIES: Pride

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20160520_CB_ColID_XX_ChiaraCalabrese_Robinson_WT_TFEB_flag1_1_1.raw Raw
20160520_CB_ColID_XX_ChiaraCalabrese_Robinson_WT_TFEB_flag1_1_2.raw Raw
20160520_CB_ColID_XX_ChiaraCalabrese_Robinson_WT_TFEB_flag1_1_3.raw Raw
20160520_CB_ColID_XX_ChiaraCalabrese_Robinson_WT_TFEB_flag1_1_4.raw Raw
20160520_CB_ColID_XX_ChiaraCalabrese_Robinson_WT_TFEB_flag1_1_5.raw Raw
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Publications

TFEB is a master regulator of autophagy, lysosome biogenesis, mitochondrial metabolism, and immunity that works primarily through transcription controlled by cytosol-to-nuclear translocation. Emerging data indicate additional regulatory interactions at the surface of organelles such as lysosomes. Here we show that TFEB has a non-transcriptional role in mitochondria, regulating the electron transport chain complex I to down-modulate inflammation. Proteomics analysis reveals extensive TFEB co-immu  ...[more]

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