Dataset Information

Peptidomics analysis of dromedary camel supraoptic nucleus by tandem mass spectrometry

ABSTRACT: Peptides were qualitatively characterized in supraoptic nuclei (SON)of dromedary camels by liquid chromatography-mass spectrometry/mass spectrometry. Samples collected in winter and summer were analyzed separately. Qualitative seasonal differences were noted. The presence of the PMCH hormone by detection of a single peptide, Neuropeptide-glutamic acid-isoleucine (EIGDEENSAKFPI-amide), only in winter SON. SCG1- and SCG2- derived peptides were detected in both seasons, but more peptides identified in winter than summer for either of the proteins. Peptides from SCG3 were detected only in winter SON samples. We found evidence of alternative splicing of the tachykinin precursor 1 in dromedary between seasons. In summer, we detected neurokinin A (isoforms 2,4, and 6) as well as peptide DADSSVEKQVALLKALYGLGQISHKMAYE confirming prohormone variant without neurokinin A (dromedary isoforms 3 and 7), while in winter SON we detected peptides supporting prohormone variants with neurokinin A (isoforms 2, 4, 6). Substance P was detected only in winter samples. The MS data supported some of our transcriptomics results.

INSTRUMENT(S): impact HD

ORGANISM(S): Camelus Dromedarius (dromedary) (arabian Camel)

TISSUE(S): Brain

SUBMITTER: Elena Romanova

LAB HEAD: Jonathan Sweedler

PROVIDER: PXD013869 | Pride | 2019-11-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Camel-SON-Summer_peptides_1_1_0.mzid.gz	Mzid
	Camel-SON-Summer_peptides_1_1_0.pride.mztab.gz	Mztab
	Camel-SON-Winter_peptides_1_1_0.mzid.gz	Mzid
	Camel-SON-Winter_peptides_1_1_0.pride.mztab.gz	Mztab
	CamelSON_S32_20160226_GB2_01_2049.d.rar	Other

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Publications

Seasonal adaptations of the hypothalamo-neurohypophyseal system of the dromedary camel.

Alim Fatma Zohra Djazouli FZD Romanova Elena V EV Tay Yea-Ling YL Rahman Ahmad Yamin Bin Abdul AYBA Chan Kok-Gan KG Hong Kar-Wai KW Rogers Mark M Southey Bruce R BR Greenwood Michael P MP Mecawi Andre Souza AS Mustafa Mohammad Rais MR Mahy Nicole N Campbell Colin C Antunes-Rodrigues José J Sweedler Jonathan V JV Murphy David D Hindmarch Charles C T CCT

PloS one 20190618 6

The "ship" of the Arabian and North African deserts, the one-humped dromedary camel (Camelus dromedarius) has a remarkable capacity to survive in conditions of extreme heat without needing to drink water. One of the ways that this is achieved is through the actions of the antidiuretic hormone arginine vasopressin (AVP), which is made in a specialised part of the brain called the hypothalamo-neurohypophyseal system (HNS), but exerts its effects at the level of the kidney to provoke water conserva ...[more]

PMID: 31211771

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