Proteomics

Dataset Information

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Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

ABSTRACT: Here we report the proteome content of exosomes released by epimastigote and bloodstream trypomastigotes forms of T. cruzi, the aetiological agent of Chagas disease. The exosomes were isolated using the ultracentrifugation method and further processed for LC-MS/MS. The results showed differential proteomic profiles of this two forms with marked differences in surface molecules cargoes. Noteworthy, the trans-sialidase (TS) surface proteins, a family of polymorphic virulence factors were differentially expressed between insect and human stages of T. cruzi highlighting the different surface nature of this two parasite stages.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Trypanosoma Cruzi Strain Cl Brener

DISEASE(S): Chagas Disease

SUBMITTER: Luis Miguel de Pablos Torró  

LAB HEAD: Dr. Antonio Osuna

PROVIDER: PXD013901 | Pride | 2021-09-08

REPOSITORIES: Pride

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Action DRS
150617_AOsuna_3.mgf Mgf
150617_AOsuna_3_Epi_3.mzid.gz Mzid
170517_AOsuna_3_Epi3.raw Raw
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Publications

Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of <i>Trypanosoma cruzi</i>.

Retana Moreira Lissette L   Prescilla-Ledezma Alexa A   Cornet-Gomez Alberto A   Linares Fátima F   Jódar-Reyes Ana Belén AB   Fernandez Jorge J   Ibarrola Vannucci Ana Karina AK   De Pablos Luis Miguel LM   Osuna Antonio A  

International journal of molecular sciences 20210513 10

Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of <i>Trypanosoma cruzi</i>, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential ce  ...[more]

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