Dataset Information

Proteomics approaches for decoding Plasmodium yoelii biology

ABSTRACT: Plasmodium yoelii is a rodent parasite commonly used as a model to study liver-stage development in host system during malaria infection. Mass spectrometry-based proteomics approaches helps in understanding the proteomic profiling of parasite and provided opportunities to explore the mechanisms controlling parasite functions. It will further help in identifying new targets for therapeutic interventions, identification of Plasmodium associated virulence in the host. It will also help in the extensive refinement of parasite genome, and understanding of Post-translational modifications (PTM) in Plasmodium yoelii biology. In the present study, we performed a proteomic shotgun analysis of the Plasmodium yoelii 17XNL strain.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Plasmodium Yoelii Yoelii 17xnl Mus Musculus (mouse)

TISSUE(S): Blood

DISEASE(S): Malaria

SUBMITTER: Keshava Prasad T. S.

LAB HEAD: T. S. Keshava Prasad

PROVIDER: PXD013932 | Pride | 2022-04-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PY17XNL_for_3f.mgf	Mgf
	Plasmodium_yoeliI_17XNL_3f_Step3.msf	Msf
	Plasmodium_yoeliI_17XNL_PTM_Step2.msf	Msf
	Plasmodium_yoeliI_17XNL_for_6f.mgf	Mgf
	Plasmodium_yoelii_17XNL_6f_Step4.msf	Msf

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Publications

Dissecting Plasmodium yoelii Pathobiology: Proteomic Approaches for Decoding Novel Translational and Post-Translational Modifications.

Rex Devasahayam Arokia Balaya DAB Patil Arun H AH Modi Prashant Kumar PK Kandiyil Mrudula Kinarulla MK Kasaragod Sandeep S Pinto Sneha M SM Tanneru Nandita N Sijwali Puran Singh PS Prasad Thottethodi Subrahmanya Keshava TSK

ACS omega 20220302 10

Malaria is a vector-borne disease. It is caused by Plasmodium parasites. Plasmodium yoelii is a rodent model parasite, primarily used for studying parasite development in liver cells and vectors. To better understand parasite biology, we carried out a high-throughput-based proteomic analysis of P. yoelii. From the same mass spectrometry (MS)/MS data set, we also captured several post-translational modified peptides by following a bioinformatics analysis without any prior enr ...[more]

PMID: 35309442

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Project description:After entering their mammalian host via the bite of an Anopheles mosquito, Plasmodium sporozoites migrate to the liver where they traverse several hepatocytes before invading the one inside which they will develop and multiply into thousands of merozoites. Although this constitutes an essential step in malaria infection, the requirements of Plasmodium parasites in liver cells and how they use the host cell for their own survival and development are poorly understood. To gain new insights into the molecular host-parasite interactions that take place during malaria liver infection, we have used high-throughput microarray technology to determine the transcriptional profile of P. yoelii-infected hepatocytes that were collected from P. yoelii-infected mice 24 and 40 h after infection. This in vivo microarray expression was compared with the microarray analysis of in vitro infected hepatoma cells infected with closely related rodent malaria parasite P. berghei. Differential expression patterns for host genes identify genes and pathways involved in the host response to rodent Plasmodium parasites. Keywords: gene expression Total RNA from sorted liver stage (LS) infected hepatocytes were isolated from PyGFP-infected BALB/c mice as described in Tarun et al (2008). RNA from LS-infected hepatocytes were isolated at two time points post-infection (pi): 24 hr pi (LS24) and 40 hr pi (LS40). As control, RNA from hepatocytes isolated from mock-infected mice (infected with salivary gland extract) at the same time points was also isolated following the same procedure. Total RNA was then subjected to two rounds of linear amplification using the Amino Allyl Message Amp II aRNA Amplification Kit (Ambion) according to manufaturerâs directions. The quality of total and amplified RNAs were examined with the Agilent 2100 BioanalyzerTM (Agilent Technologies) and the quantity of the RNA samples was assessed using a Nanodrop ND-1000 spectrophotometer prior to microarray hybridization. For each timepoint two independent biological replicates were obtained.

Dataset Information

Proteomics approaches for decoding Plasmodium yoelii biology

Dataset's files

Publications

Dissecting <i>Plasmodium yoelii</i> Pathobiology: Proteomic Approaches for Decoding Novel Translational and Post-Translational Modifications.

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