Dataset Information

NanoLC-MSMS Phosphoproteomics comparison of in vitro cultured reticulocytes and red blood cells before, during and after microsphiltration

ABSTRACT: Four samples of in vitro culture-derived reticulocytes (biological replicates, derived from CD34+ cells) and three samples of red blood cells (biological replicates) were analysed through qualitative phosphoproteomics before, during and after passage through a spleen-mimicking microsphiltration device in order to investigate the mechanisms underlying shear stress recognition.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Blood Cell, Reticulocyte, Blood

SUBMITTER: Pedro Moura

LAB HEAD: Ashley Mark Toye

PROVIDER: PXD013960 | Pride | 2019-09-12

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	150817_Retics_Mid.mgf	Mgf
	150817_Retics_Mid.pride.mgf.gz	Mgf
	150817_Retics_Post.mgf	Mgf
	150817_Retics_Post.pride.mgf.gz	Mgf
	150817_Retics_Pre.mgf	Mgf

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Publications

Reticulocyte and red blood cell deformation triggers specific phosphorylation events.

Moura Pedro L PL Lizarralde Iragorri Maria A MA Français Olivier O Le Pioufle Bruno B Dobbe Johannes G G JGG Streekstra Geert J GJ El Nemer Wassim W Toye Ashley M AM Satchwell Timothy J TJ

Blood advances 20190901 17

The capacity to undergo substantial deformation is a defining characteristic of the red blood cell (RBC), facilitating transit through the splenic interendothelial slits and microvasculature. Establishment of this remarkable property occurs during a process of reticulocyte maturation that begins with egress through micron-wide pores in the bone marrow and is completed within the circulation. The requirement to undertake repeated cycles of deformation necessitates that both reticulocytes and eryt ...[more]

PMID: 31506283

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