Proteomics

Dataset Information

0

Histone octamer structure is altered early in ISW2 ATP-dependent remodeling of nucleosomes

ABSTRACT: Histone octamers are thought to be a rigid part of nucleosomes that shape chromatin and block cellular machinery from accessing DNA. ATP-dependent chromatin remodelers like ISW2 mobilize nucleosomes to provide DNA access. We find evidence for histone octamer distortion preceding DNA being moved into nucleosomes and processive movement of the ATPase motor of ISW2 on nucleosomal DNA. DNA entering nucleosome is uncoupled from the ATPase activity of ISW2 and alterations of the histone octamer structure mediated by ISW2 by deletion of the SANT domain from the C-terminus of the Isw2 catalytic subunit. We also find that restricting histone movement by chemical crosslinking traps remodeling intermediates resembling those seen by loss of the SANT domain, further supporting the importance of changes in histone octamer structure early in ISW2 remodeling. Transient octamer distortions are stabilized by H3-H4 tetramer disulfide crosslinking, thereby linking intrinsic histone octamer flexibility to chromatin remodeling.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

SUBMITTER: Jie Luo  

LAB HEAD: Jeff Ranish

PROVIDER: PXD013967 | Pride | 2020-05-27

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
BS3.inter1.xls Xls
BS3.intra1.xls Xls
JL032717_032817_NCP_DISH_100D.mzXML Mzxml
JL032717_032817_NCP_DISH_50B.mzXML Mzxml
JL032717_032817_NCP_DISH_5D.mzXML Mzxml
Items per page:
1 - 5 of 21
altmetric image

Publications

Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling.

Hada Arjan A   Hota Swetansu K SK   Luo Jie J   Lin Yuan-Chi YC   Kale Seyit S   Shaytan Alexey K AK   Bhardwaj Saurabh K SK   Persinger Jim J   Ranish Jeff J   Panchenko Anna R AR   Bartholomew Blaine B  

Cell reports 20190701 1

Nucleosomes are the fundamental building blocks of chromatin that regulate DNA access and are composed of histone octamers. ATP-dependent chromatin remodelers like ISW2 regulate chromatin access by translationally moving nucleosomes to different DNA regions. We find that histone octamers are more pliable than previously assumed and distorted by ISW2 early in remodeling before DNA enters nucleosomes and the ATPase motor moves processively on nucleosomal DNA. Uncoupling the ATPase activity of ISW2  ...[more]

Similar Datasets

Proteomics TAF8 regions important for TFIID lobe B assembly are required for embryonic stem cell survival
2021-10-02 | PXD026575 | Pride
Proteomics SWI/SNF is positively auto-regulated by an AT-hook in the catalytic subunit
2023-06-15 | PXD040664 | Pride
Proteomics Unique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex
2024-08-08 | PXD053209 | Pride
Proteomics BRCA1/BARD1 intrinsically disordered regions facilitate chromatin recruitment and ubiquitylation
2023-06-21 | PXD035345 | Pride
Proteomics,Multiomics Yeast SWI/SNF complex - Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex
2018-06-14 | PXD005948 | Pride
Proteomics Human BAF complex (HA-DPF2 pulldown and SS18-Flag pulldown)
2018-10-18 | PXD010124 | Pride
Proteomics Human pBAF complex (HA-BRD7, PHF10-HA pulldown)
2018-10-18 | PXD010122 | Pride
Proteomics Drosophila BAF complex crosslinking (
2018-10-18 | PXD010123 | Pride
Proteomics Crosslinking RNF168(1-113) to Nucleosome core particels
2019-04-02 | PXD012723 | Pride
Proteomics Crosslinking Mapping of yeast Komagataella phaffii TFIID complex
2018-10-08 | PXD011092 | Pride