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Secretome analysis of Knufia chersonesos MA5789, wt and MA5790 mutant

ABSTRACT: The extremotolerant rock-associated black fungus Knufia chersonesos and its nonmelanized spontaneous mutant were selected for a proteomic-based screening towards polyesterases. A non-labeling shotgun analysis of the secretome was performed to compare control and treatment condition (PBAT added to the cultivation media) as well as Wt and Mut.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Knufia Petricola

SUBMITTER: Donatella Tesei

LAB HEAD: Donatella Tesei

PROVIDER: PXD014026 | Pride | 2020-06-24

REPOSITORIES: Pride

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Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos.

Tesei Donatella D Quartinello Felice F Guebitz Georg M GM Ribitsch Doris D Nöbauer Katharina K Razzazi-Fazeli Ebrahim E Sterflinger Katja K

Scientific reports 20200617 1

Knufia chersonesos is an ascomycotal representative of black fungi, a morphological group of polyextremotolerant melanotic fungi, whose ability to resort to recalcitrant carbon sources makes it an interesting candidate for degradation purposes. A secretome screening towards polyesterases was carried out for the fungus and its non-melanized mutant, grown in presence of the synthetic copolyester Polybutylene adipate terephthalate (PBAT) as additional or sole carbon source, and resulted in the iden ...[more]

PMID: 32555357

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