Proteomics

Dataset Information

0

Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons


ABSTRACT: At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling strategy in cultured hippocampal neurons stimulated with DHPG, a specific agonist for group I mGluRs. We identified several kinases with important roles in DHPG-induced mGluR activation, which we confirmed using small molecule kinase inhibitors. Furthermore, changes in the AMPA receptor endocytosis pathway in both protein synthesis and protein phosphorylation were identified, whereby Intersectin-1 was validated as a novel player in this pathway. This study revealed several new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and provides a rich resource for further analyses.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Hippocampal Neuron, Cell Culture

SUBMITTER: Charlotte van Gelder  

LAB HEAD: Maarten Altelaar

PROVIDER: PXD014043 | Pride | 2020-09-11

REPOSITORIES: Pride

altmetric image

Publications

Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons.

van Gelder Charlotte A G H CAGH   Penning Renske R   Veth Tim S TS   Catsburg Lisa A E LAE   Hoogenraad Casper C CC   MacGillavry Harold D HD   Altelaar Maarten M  

Molecular & cellular proteomics : MCP 20200910 12


At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitativ  ...[more]

Similar Datasets

2013-12-31 | E-GEOD-22993 | biostudies-arrayexpress
2012-01-03 | E-GEOD-22465 | biostudies-arrayexpress
2013-01-23 | E-GEOD-22087 | biostudies-arrayexpress
2015-09-08 | E-GEOD-66279 | biostudies-arrayexpress
2010-04-09 | E-GEOD-16223 | biostudies-arrayexpress
2011-03-07 | E-GEOD-18860 | biostudies-arrayexpress
2021-12-20 | PXD025730 | Pride
2022-05-16 | E-MTAB-11452 | biostudies-arrayexpress
2016-09-29 | PXD004744 | Pride
2021-06-28 | PXD026289 | Pride