Dataset Information

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

ABSTRACT: Analysis of proteins under the influence of the quorum sensing (QS) system in the nonpathogenic Agrobacterium tumefaciens strain 6N2, and the influence of the bacterial QS system in the proteome of the yeast Meyerozyma guilliermondii strain 6N.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rhizobium Radiobacter (agrobacterium Tumefaciens) (agrobacterium Radiobacter) Meyerozyma Guilliermondii

SUBMITTER: Carlos Nieto Peñalver

LAB HEAD: Carlos Nieto-Peñalver

PROVIDER: PXD025730 | Pride | 2021-12-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	355281-1615001-ATQSplus-CG-ATQSmoins-CG-CG-alone.msf	Msf
	355281-spectra.mgf	Mgf
	488425-1615001-ATQSplus-ATQSmoins-ATQSplus-ATQSmoins-CG.msf	Msf
	488425-spectra.mgf	Mgf
	ATQS-plus-ATQS-moins-ATQS-plus-CG-ATQS-moins-CG-488425-spectra.xlsx	Xlsx

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Publications

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii.

Bertini Elisa Violeta EV Torres Mariela Analía MA Léger Thibaut T Garcia Camille C Hong Kar-Wai KW Chong Teik Min TM Castellanos de Figueroa Lucía I LI Chan Kok-Gan KG Dessaux Yves Y Camadro Jean-Michel JM Nieto-Peñalver Carlos Gabriel CG

Genomics 20211115 6

Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OH ...[more]

PMID: 34793950

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Project description:Quorum-sensing (QS) is a cell-cell communication system that controls gene expression in many bacterial species, mediated by diffusible signal molecules. While the intracellular regulatory mechanisms of QS are often well-understood, the functional roles of QS remain controversial. In particular, the use of multiple signals by many bacterial species poses a serious challenge to current functional theories. Here we address this challenge by showing that bacteria can use multiple QS signals to infer both their social (density) and physical (mass-transfer) environment. Analytical and evolutionary simulation models show that the detection of and response to complex social/physical contrasts requires multiple signals with distinct half-lives and combinatorial (non-additive) responses to signal concentrations. We test these predictions using the opportunistic pathogen Pseudomonas aeruginosa, and demonstrate significant differences in signal decay between its two primary signal molecules as well as diverse combinatorial responses to dual signal inputs. QS is associated with the control of secreted factors, and we show that secretome genes are preferentially controlled by synergistic M-bM-^@M-^XAND-gateM-bM-^@M-^Y responses to multiple signal inputs, ensuring the effective expression of secreted factors in high density and low mass-transfer environments. Our results support a novel functional hypothesis for the use of multiple signals and, more generally, show that bacteria are capable of combinatorial communication. The two primary signal molecules of P. aeruginosa are the homoserine lactones N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL) and N-butyryl-homoserine lactone (C4-HSL). Effects of the different signal molecules was assessed using a double QS synthase mutant of Pseudomonas aeruginosa PAO1 lasI/rhlI grown at 37M-BM-0C in 25 ml LB broth and 250 ml flasks with shaking at 200 r.p.m. in four treatments, each with a replicate: (a) no addition; (b) 3-oxo- C12-HSL; (c) C4-HSL; and (d) both 3-oxo-C12-HSL and C4-HSL.