Proteomics

Dataset Information

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Human placental tissue LC-MS/MS


ABSTRACT: This project aimed at missing protein identification from human placental tissue, combining sample preparation and mass spectrometry technology development.For sample preparation, low molecular proteins were enriched by C18 solid phase extraction, SDS-PAGE and MWCO filter. For LC-MS/MS analysis, micro-HPLC fractionation was carried out coupled with LUMOS instrument. Proteome Discoverer 2.2 software was used for data analysis to identify missing proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Placenta

SUBMITTER: yuanliang zhang  

LAB HEAD: Siqi Liu

PROVIDER: PXD014083 | Pride | 2019-10-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
placenta_C18_1_1-30frac.pep.xml Pepxml
placenta_C18_1_1-30frac_semi.pep.xml Pepxml
placenta_C18_1_F1.raw Raw
placenta_C18_1_F10.raw Raw
placenta_C18_1_F11.raw Raw
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Publications

Alternative Strategy To Explore Missing Proteins with Low Molecular Weight.

Lin Zhilong Z   Zhang Yuanliang Y   Pan Huozhen H   Hao Piliang P   Li Siqi S   He Yanbin Y   Yang Huanming H   Liu Siqi S   Ren Yan Y  

Journal of proteome research 20191108 12


Identifying more missing proteins (MPs) is an important mission of C-HPP. With the number of identified MPs being attenuated year by year (2,949 to 2,129 MPs from 2016 to 2019), we have realized that the difficulty of exploring the remaining MPs is a challenge in technique. Herein, we propose a comprehensive strategy to effectively enrich, separate, and identify proteins with low molecular weights, aiming at the discovery of MPs. Basically, a protein extract from human placenta was passed throug  ...[more]

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