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Optimization of label-free nano LC-MS/MS analysis of the placental proteome

ABSTRACT: We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Placenta

SUBMITTER: Marc Dieu

LAB HEAD: Florence CHAINIAUX-DEBACQ

PROVIDER: PXD020438 | Pride | 2020-10-13

REPOSITORIES: Pride

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			Action	DRS
	281474976714517F049083.mzid.gz	Mzid
	281474976714517F049083.mzid_281474976714517_F049083.MGF	Mzid
	281474976714518F049084.mzid.gz	Mzid
	281474976714518F049084.mzid_281474976714518_F049084.MGF	Mzid
	281474976714572F049138.mzid.gz	Mzid

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Optimization of label-free nano LC-MS/MS analysis of the placental proteome.

Luyten Leen J LJ Dieu Marc M Demazy Catherine C Fransolet Maude M Nawrot Tim S TS Renard Patricia P Debacq-Chainiaux Florence F

Placenta 20200914

The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a s ...[more]

PMID: 32992125

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