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Age-dependent dynamics of ribosomal proteins in mouse brain

ABSTRACT: To determine the dynamics of ribosomal proteins in brain tissues, we isolated monoribosomes and ribosomes engaged in polysomal fractions from three different brain tissues, cerebellum, hippocampus and cortex, and compared them to the liver of CD-1 IGS mouse. We provide a comprehensive unique study of the ribosomal composition among these different tissues along different life stages, from juvenile (three-weeks), young adult (four-months) to mature adults (seven- and twelve-months) of age. The protein composition of the ribosomes was quantified using LC-MS/MS approach.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Christoph Krisp

LAB HEAD: Zoya Ignatova

PROVIDER: PXD014138 | Pride | 2019-10-10

REPOSITORIES: Pride

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			Action	DRS
	180112_PK_SA_DIA_01a.mzML	Mzml
	180112_PK_SA_DIA_01a.raw	Raw
	180112_PK_SA_DIA_01b.mzML	Mzml
	180112_PK_SA_DIA_01b.raw	Raw
	180112_PK_SA_DIA_02a.mzML	Mzml

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Publications

Invariable stoichiometry of ribosomal proteins in mouse brain tissues with aging.

Amirbeigiarab Susan S Kiani Parnian P Velazquez Sanchez Ana A Krisp Christoph C Kazantsev Andriy A Fester Lars L Schlüter Hartmut H Ignatova Zoya Z

Proceedings of the National Academy of Sciences of the United States of America 20191021 45

Across phyla, the ribosomes-the central molecular machines for translation of genetic information-exhibit an overall preserved architecture and a conserved functional core. The natural heterogeneity of the ribosome periodically phases a debate on their functional specialization and the tissue-specific variations of the ribosomal protein (RP) pool. Using sensitive differential proteomics, we performed a thorough quantitative inventory of the protein composition of ribosomes from 3 different mouse ...[more]

PMID: 31636180

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Increased sucrose levels mediate selective mRNA translation in Arabidopsis

Project description:Background Protein synthesis is a highly energy demanding process and is regulated according to energy availability in plant cells. Light and sugar availability affect mRNA translation but the specific roles of these factors remain unclear. In this study, sucrose was applied to Arabidopsis seedlings kept in the light or in the dark, in order to distinguish sucrose and light effects on transcription and translation. These were studied using microarray analysis of steady state mRNA and mRNA bound to translating ribosomes. Results Steady state mRNA levels were affected differently by sucrose in the light and in the dark but general translation increased to a similar extend in both conditions. Alterations in polysomal mRNA association closely followed the changes induced on the transcript level. However, for 243 mRNAs, a change in ribosome occupancy was observed after sucrose treatment in the light, but not in the dark condition. Many of these mRNAs are annotated as encoding ribosomal proteins, supporting specific translational regulation of this group of transcripts. Unexpectedly, the numbers of ribosomes bound to each mRNA decreased for mRNAs with increased ribosome occupancy. Conclusions Our results suggest that sucrose regulate translation of these 243 mRNAs but specifically in the light, through a novel regulatory mechanism. Our data sows that increased polysomal association is not necessarily leading to more ribosomes per transcript, suggesting a mechanism of translational induction not solely dependent on increased translation initiation rates. Four different samples groups were used: Control, Darkness, Sucrose, Simultaneous Darkness and sucrose treatments. All sample groups was represented by both total mRNA hybridization and of mRNAs from polysomal enrichment. All sample groups were represented by three biological replicates.

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