Dataset Information

Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching

ABSTRACT: Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have isolated ribosomal complexes and characterised their protein content across 4 tissues of Drosophila melanogaster via TMT-MS. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of ribosomal protein paralog-enrichment and ribosomal protein paralog-switching. We have also solved structures of ribosomes isolated from tissues by cryo-EM, revealing differences in precise ribosomal arrangement for testis and ovary 80S ribosomes. Differences in the amino acid composition of paralog pairs and their localisation on the ribosome exterior indicate paralog-switching could alter the ribosome surface, enabling different proteins to regulate translation. One testis-specific paralog-switching pair is also found in humans, suggesting this is a conserved site of ribosome heterogeneity. Overall, this work allows us to propose that mRNA translation might be regulated in the gonads through ribosome heterogeneity, providing a potential means of ribosome specialisation.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Testis, Ovary, Head, Embryo

SUBMITTER: Karl Norris

LAB HEAD: Julie L Aspden

PROVIDER: PXD026227 | Pride | 2021-07-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	TMT1_280218_Fraction_1.raw	Raw
	TMT1_280218_Fraction_2.raw	Raw
	TMT1_280218_Fraction_3.raw	Raw
	TMT1_280218_Fraction_4.raw	Raw
	TMT1__280218_5Plex_TMT_RptAnalysis050121_PS.msf	Msf

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Publications

Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.

Hopes Tayah T Norris Karl K Agapiou Michaela M McCarthy Charley G P CGP Lewis Philip A PA O'Connell Mary J MJ Fontana Juan J Aspden Julie L JL

Nucleic acids research 20220201 4

Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have characterised ribosomal protein heterogeneity across 4 tissues of Drosophila melanogaster. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of paralog-enrichment and paralog-switching. We have solved structures of ribosomes purified from ...[more]

PMID: 34283226

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