Proteomics

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Integrated analyses of shotgun proteomics and metabolomics reveal its association with rise of secondary metabolites of warm water imbibition soybean seeds

ABSTRACT: For production of soy-foods or supplements, imbibition of soybean seeds in the water is required step for generation of tofu, soy-milk, and other soy-products. With an aim to get new insight into effects of different imbibition temperature (4, 25, and 55℃), this study conducted integrated proteomics and metabolomics analysis of soybean seeds. For total proteome analysis, we applied TMT labeling based quantitative proteomics combined to FASP (Filter-Aided Sample Preparation) with high-throughput LC-MS/MS. A total of 2,616 proteins were identified out of which 801 proteins showed significantly difference of protein abundance (≥1.5 fold change, Benjamini-Hochberg FDR <0.05) among 4, 25, and 55℃ imbibition seeds. Functional analysis of identified proteins showed an increased abundance of proteins functioning as glycosyl hydrolase enzymes such as beta-glucosidase, alpha and beta-galactosidase, and alpha-mannosidase, or protease, and PTMs related enzymes as well. UPLC TOF-MS analysis showed around 20 fold increase in isoflavone aglycones (daidzein and genistein) while isoflavone glycosides (daidzin and genistin) were decreased in 55℃ imbibition seed, in agreement with proteomics results which we assume positively related to increase abundance of glycosyl hydrolase. A metabolomics analysis revealed 64 metabolites were significantly altered, for example, various free amino acids showed accumulation patterns by increased abundance of various protease enzymes and further confirmed the accumulation of isoflavone aglycones and degradation of raffinose and stachyose in 55℃ imbibition seeds. Based on these results, we recommend the use of 55℃ for soybean seed imbibition to increase the quality of soy-food products.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Glycine Max

TISSUE(S): Seed

SUBMITTER: Sun Tae Kim  

LAB HEAD: Sun Tae Kim

PROVIDER: PXD014152 | Pride | 2020-09-25

REPOSITORIES: Pride

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Publications

Integrated Proteomics and Metabolomics Analysis Highlights Correlative Metabolite-Protein Networks in Soybean Seeds Subjected to Warm-Water Soaking.

Min Cheol Woo CW   Hyeon Hyejin H   Gupta Ravi R   Park Joonho J   Cheon Ye Eun YE   Lee Gi Hyun GH   Jang Jeong Woo JW   Ryu Hyung Won HW   Lee Byong Won BW   Park Sang Un SU   Kim Youngsoo Y   Kim Jae Kwang JK   Kim Sun Tae ST  

Journal of agricultural and food chemistry 20200715 30

Soaking of soybean seeds is a prerequisite for the production of soy foods, and it has been shown that the extent of water absorbed during different imbibition conditions directly affects the quality of the subsequent soybean seed products by yet unknown mechanisms. In order to elucidate the molecular changes in soybean seeds during different soaking temperatures, we performed an integrated proteomics and metabolomics analysis of seeds soaked at 4, 25, and 55 °C. Proteomics analysis revealed tha  ...[more]

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