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Top-down characterization of lunasin in a commercial soybean-derived product

ABSTRACT: The presence of lunasin was investigated in two soybean products, raw soy seeds and a commercial soybean beverage powder. Lunasin from the commercial soybean derivative was purified by reverse phase high pressure liquid chromatography and bystrong anion exchange solid phase extraction. Lunasin was further characterized by Top-down mass spectrometry (MS). The top-down characterization of lunasin unraveled a wide range of post-translationally modified proteoforms.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Glycine Max

TISSUE(S): Seed

SUBMITTER: Aida Serra

LAB HEAD: Siu Kwan Sze

PROVIDER: PXD003064 | Pride | 2016-05-24

REPOSITORIES: Pride

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			Action	DRS
	141201_SBPowder_Lunasin_EA_SAX_C18_A.raw	Raw
	141201_SBPowder_Lunasin_EA_SAX_C18_B.raw	Raw
	141201_SBPowder_Lunasin_EA_SAX_C18_C.raw	Raw
	141201_Soybean_powder_EA_30KDa_SAX_C18_141201220209.raw	Raw
	150327_Lunasin.raw	Raw

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Publications

Commercial processed soy-based food product contains glycated and glycoxidated lunasin proteoforms.

Serra Aida A Gallart-Palau Xavier X See-Toh Rachel Su-En RS Hemu Xinya X Tam James P JP Sze Siu Kwan SK

Scientific reports 20160518

Nutraceuticals have been proposed to exert positive effects on human health and confer protection against many chronic diseases. A major bioactive component of soy-based foods is lunasin peptide, which has potential to exert a major impact on the health of human consumers worldwide, but the biochemical features of dietary lunasin still remain poorly characterized. In this study, lunasin was purified from a soy-based food product via strong anion exchange solid phase extraction and then subjected ...[more]

PMID: 27189269

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