Dataset Information

The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus

ABSTRACT: Daphnia infochemicals can induce defensive responses in microalgae Scenedesmus spp., including colony formation and flocculation. We investigated the phenomenon using a combination of microalgal biology, physiological ecology, and quantitative isobaric tagging (iTRAQ) proteomics. We have discovered that flocculation as a defense response occurs at the early ‘alarm’ phase and requires increased energy resources, with an important role proposed in cysteine synthesis. It appears to be initially stimulated by the production of an extracellular matrix (mainly composed ofby polysaccharides and fatty acids), and later sustained at the ‘acclimation’ stage through Mitogen-activated protein kinase (MAPK) signalling cascades. Defensive strategies leading to colony formation required investment into fatty acids metabolism, to ensure the accurate separation of membranes during cell division. Furthermore, higher energy demands were required at the alarm phase which subsequently decreased at the acclimation stage, thus suggesting a trade-off between colony formation and support of floc form.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Desmodesmus Subspicatus

TISSUE(S): Cell Suspension Culture

SUBMITTER: Sebastiana Roccuzzo

LAB HEAD: Jagroop Pandhal

PROVIDER: PXD014153 | Pride | 2020-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_Peptide_MSMS_iTRAQ_20h_acclimation_phase.xlsx	Xlsx
	MaxQuant_ProteinGroups_iTRAQ_20h_acclimation_phase.xlsx	Xlsx
	MicroalgaeRef_merged_file_validated.fasta	Fasta
	ProteinGroups_alarm_phase.xlsx	Xlsx
	iTRAQ20h.zip	Other

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Publications

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Scenedesmus subspicatus Unraveled by Quantitative Proteomics.

Roccuzzo Sebastiana S Couto Narciso N Karunakaran Esther E Kapoore Rahul Vijay RV Butler Thomas O TO Mukherjee Joy J Hansson Erika M EM Beckerman Andrew P AP Pandhal Jagroop J

Frontiers in microbiology 20200507

Microalgae can respond to natural cues from crustacean grazers, such as Daphnia, by forming colonies and aggregations called flocs. Combining microalgal biology, physiological ecology, and quantitative proteomics, we identified how infochemicals from Daphnia trigger physiological and cellular level changes in the microalga Scenedesmus subspicatus, underpinning colony formation and flocculation. We discovered that flocculation occurs at an energy-demanding 'alarm' phase, with ...[more]

PMID: 32457714

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Project description:Anthocyanins and flavonols are natural compounds that accumulate preferentially in grapevine flowers and fruits. They are among the most abundant flavonoids and play a very important role in grape and wine quality. In particular, they confer and stabilize colour and contribute to other organoleptic characteristics of the final product. Their complex profile in terms of concentration and relative abundance varies among cultivars and makes wine typicity. The synthesis of these compounds is mainly regulated at transcriptional level. Flavonoids accumulate at specific stages and in specific tissues during flower and berry development, as a consequence of the timely expression of the genes necessary for their synthesis. Although the general flavonoid pathway has been genetically and biochemically elucidated and the main determinants of colour have been identified, the molecular reasons of the fine variation among grape cultivars are still not completely understood. To shed light on this issue, extreme genotypes of a segregating population derived from the cross Syrah x Pinot Noir were characterized at transcriptional level. The transcriptome analysis along three berry developmental stages of these genotypes has allowed the identification of a large set of transcripts differentially modulated between the two groups. A population derived from M-bM-^@M-^XSyrahM-bM-^@M-^Y x M-bM-^@M-^XPinot NoirM-bM-^@M-^Y consisting of 170 F1 individuals plus the parental lines was characterized for the content of flavonols and anthocyanins in the skins of mature berries (38E-L; 18M-BM-0Brix). Based on the biochemical data, 4 high- and 4 low-flavonol producers (HFPs and LFPs: F1 16, F1 56, F1 63, F1 223 and F1 64, F1 256, F1 260, PN), two of which having also either very high or very low anthocyanin content (HAPs and LAPs: F1 63, F1 223 and F1 256, F1 260), were selected for gene expression analysis. A representative sample for each individual (one biological replicate) was collected at three berry developmental stages (hard green berry (33E-L, PV), veraison (35E-L, 50% coloured berries, VER) and maturity (38E-L, 18M-BM-0Brix, MAT)) during the 2007 season.

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Dataset Information

The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus

Dataset's files

Publications

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in <i>Scenedesmus subspicatus</i> Unraveled by Quantitative Proteomics.

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