Dataset Information

Proteomics of blood sera from Macaca fascicularis in the framework of the development of a vaccine against Lassa virus.

ABSTRACT: Next-generation proteomics of blood sera from Macaca fascicularis monkeys immunized with measles-based and Mopeia-based vaccine platforms.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Macaca Fascicularis (crab-eating Macaque) (cynomolgus Monkey)

TISSUE(S): Blood Plasma

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD014277 | Pride | 2020-02-25

REPOSITORIES: pride

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Publications

Vaccines inducing immunity to Lassa virus glycoprotein and nucleoprotein protect macaques after a single shot.

Mateo Mathieu M Reynard Stéphanie S Carnec Xavier X Journeaux Alexandra A Baillet Nicolas N Schaeffer Justine J Picard Caroline C Legras-Lachuer Catherine C Allan Richard R Perthame Emeline E Hillion Kenzo-Hugo KH Pietrosemoli Natalia N Dillies Marie-Agnès MA Barrot Laura L Vallve Audrey A Barron Stéphane S Fellmann Lyne L Gaillard Jean-Charles JC Armengaud Jean J Carbonnelle Caroline C Raoul Hervé H Tangy Frédéric F Baize Sylvain S

Science translational medicine 20191001 512

Lassa fever is a major threat in Western Africa. The large number of people living at risk for this disease calls for the development of a vaccine against Lassa virus (LASV). We generated live-attenuated LASV vaccines based on measles virus and Mopeia virus platforms and expressing different LASV antigens, with the aim to develop a vaccine able to protect after a single shot. We compared the efficacy of these vaccines against LASV in cynomolgus monkeys. The vaccines were well tolerated and prote ...[more]

PMID: 31578242

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Project description:The long-tailed macaque, also referred to as cynomolgus monkey (Macaca fascicularis), is one of the most important non-human primate animal models in basic and applied biomedical research. To improve the predictive power of primate experiments for humans, we determined the genome sequence of a Macaca fascicularis female of Mauritian origin using a whole-genome shotgun sequencing approach. We applied a template switch strategy which employs either the rhesus or the human genome to assemble sequence reads. The 6-fold sequence coverage of the draft genome sequence enabled discovery of about 2.1 million potential single-nucleotide polymorphisms based on occurrence of a dimorphic nucleotide at a given position in the genome sequence. Homology-based annotation allowed us to identify 17,387 orthologs of human protein-coding genes in the M. fascicularis draft genome and the predicted transcripts enabled the design of a M. fascicularis-specific gene expression microarray. Using liver samples from 36 individuals of different geographic origin, we identified 718 genes with highly variable expression in liver, whereas the majority of the transcriptome shows relatively stable and comparable expression. Knowledge of the M. fascicularis draft genome is an important contribution to both the use of this animal in disease models and the safety assessment of drugs and their metabolites. In particular, this information allows high-resolution genotyping and microarray-based gene expression profiling for animal stratification, thereby allowing the use of well-characterized animals for safety testing. Finally, the genome sequence presented here is a significant contribution to the global "3R" animal welfare initiative, which has the goal to reduce, refine and replace animal experiments. A 36-microarray study using total RNA recovered from liver samples of untreated Cynomolgus monkeys of good laboratory practice (GLP) drug safety studies. The monkeys were from the Philippines, a Chinese colony, and Mauritius. Each microarray measures the expression level of 16,896 genes using 20,047 probe sets with six 60-mer probes (PM) per probe set. Each probe set is represented once on the array. The Cynomolgus monkey gene expression results analyzed in this study are further described in Ebeling et al. (2011) (PMID 21862625).

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