Dataset Information

PepSAVI-MS reveals proline-rich antimicrobial peptide in Amaranthus tricolor

ABSTRACT: PepSAVI-MS, a mass spectrometry-based peptidomics pipeline, was implemented for antimicrobial peptide (AMP) discovery in the medicinal plant Amaranthus tricolor. This investigation revealed a novel 1.7 kDa AMP, deemed Atr-AMP1. Initial efforts to determine the sequence of Atr-AMP1 utilized chemical derivatization and enzymatic digestion to provide information about specific residues and post-translational modifications. EThcD (electron-transfer/higher-energy collision dissociation) produced extensive backbone fragmentation and facilitated de novo sequencing, the results of which were consistent with orthogonal characterization experiments. Additionally, multistage HCD (higher-energy collisional dissociation) facilitated discrimination between isobaric leucine and isoleucine. These results revealed a positively-charged proline-rich peptide present in a heterogeneous population of multiple peptidoforms, possessing several post-translational modifications including a disulfide bond, methionine oxidation, and proline hydroxylation.

INSTRUMENT(S): Orbitrap Fusion Lumos, TripleTOF 5600

ORGANISM(S): Amaranthus Tricolor

TISSUE(S): Leaf

SUBMITTER: Tessa Bartges

LAB HEAD: Leslie Hicks

PROVIDER: PXD014547 | Pride | 2020-05-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180308_CLK_BAP_ATr_SCX3_28.wiff	Wiff
	20180308_CLK_BAP_ATr_SCX3_28.wiff.scan	Wiff
	20180315_CLK_BAP_ATr_SCX3_Frac28_RA.wiff	Wiff
	20180315_CLK_BAP_ATr_SCX3_Frac28_RA.wiff.scan	Wiff
	20180326_CLK_BAP_ATr_RAD_DDA_RAD_Trypsin.wiff	Wiff

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Publications

Design, Carbon Emission and Economic Analysis of a Cogeneration System for Garments Group of Industries

Jain Narayan Lal NL

20221104

Cogeneration is the most efficient and reliable energy supply technology which is being utilized worldwide in individual industries but not in a group of industries. This paper presents a study which has been carried out to check the feasibility and economic viability of Combined Heat and Power Plant (CHP) in a group of industries of garments zone. Heat and power forms of energy are required in existing system. Electricity is supplied by the state grid while heat in the form of steam is generate ...[more]

PMID: PPR567511

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