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Identification and characterization of CC-AMP1-like and CC-AMP2-like peptides in Capsicum spp.

ABSTRACT: Antimicrobial peptides (AMPs) are compounds with a variety of bioactive properties. Especially promising are their antibacterial activities, often towards drug-resistant pathogens. Across different AMP sources, AMPs expressed within plants are relatively underexplored, with a limited number of plant AMP families identified. Recently, we identified the novel AMPs CC-AMP1 and CC-AMP2 in ghost pepper plants (Capsicum chinense x frutescens), exerting promising antibacterial activity and not classifying into any known plant AMP family. Herein, AMPs related to CC-AMP1 and CC-AMP2 were identified within both Capsicum annuum and Capsicum baccatum. Targeted MS/MS experiments were performed to determine peptide sequences, guided by in silico AMP sequence predictions.

INSTRUMENT(S): Q Exactive HF-X, TripleTOF 5600

ORGANISM(S): Capsicum Baccatum Capsicum Annuum

TISSUE(S): Leaf

SUBMITTER: Leslie Hicks

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD041035 | Pride | 2024-01-27

REPOSITORIES: Pride

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			Action	DRS
	20210413_KDC_CA_100x_1uL_26.wiff	Wiff
	20210413_KDC_CA_100x_1uL_26.wiff.scan	Wiff
	20210413_KDC_CA_100x_1uL_27.wiff	Wiff
	20210413_KDC_CA_100x_1uL_27.wiff.scan	Wiff
	20210413_KDC_CA_100x_1uL_29.wiff	Wiff

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Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper

Project description:Capsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions are paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense x frutescens). AMP prediction algorithms reveal 115 putative AMPs within the Capsicum chinense genome of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of post-translational modifications and disulfide bond connectivity. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibit low µM antimicrobial activity against gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to more fully characterize plant AMP profiles.

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