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Endoplasmic Reticulum Chaperones Stabilize Ligand-Receptive MR1 molecules for Efficient Presentation of Metabolite Antigens

ABSTRACT: Quantitative MS analysis of MR1 GFP immunoprecipates to identify steady-state binding partnets.

INSTRUMENT(S): impact II

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Laura Dagley

LAB HEAD: Hamish E.G. McWilliam

PROVIDER: PXD014585 | Pride | 2020-09-16

REPOSITORIES: pride

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	P3197_CTRL_1_1_1_1_01_4822.d.zip	Other
	P3197_CTRL_1_2_1_1_01_4823.d.zip	Other
	P3197_CTRL_1_3_1_1_01_4824.d.zip	Other
	P3197_NL_1_1_1_1_01_4826.d.zip	Other
	P3197_NL_1_2_1_1_01_4827.d.zip	Other

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Publications

Endoplasmic reticulum chaperones stabilize ligand-receptive MR1 molecules for efficient presentation of metabolite antigens.

McWilliam Hamish E G HEG Mak Jeffrey Y W JYW Awad Wael W Zorkau Matthew M Cruz-Gomez Sebastian S Lim Hui Jing HJ Yan Yuting Y Wormald Sam S Dagley Laura F LF Eckle Sidonia B G SBG Corbett Alexandra J AJ Liu Haiyin H Li Shihan S Reddiex Scott J J SJJ Mintern Justine D JD Liu Ligong L McCluskey James J Rossjohn Jamie J Fairlie David P DP Villadangos Jose A JA

Proceedings of the National Academy of Sciences of the United States of America 20200921 40

The antigen-presenting molecule MR1 (MHC class I-related protein 1) presents metabolite antigens derived from microbial vitamin B<sub>2</sub> synthesis to activate mucosal-associated invariant T (MAIT) cells. Key aspects of this evolutionarily conserved pathway remain uncharacterized, including where MR1 acquires ligands and what accessory proteins assist ligand binding. We answer these questions by using a fluorophore-labeled stable MR1 antigen analog, a conformation-specific MR1 mAb, proteomic ...[more]

PMID: 32958637

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