Proteomics

Dataset Information

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PROTEOMIC ANALYSIS OF EXTRACELLULAR VESICLES FROM RHEUMATOID ARTHRITIS SYNOVIAL FLUID

ABSTRACT: Previously, extracellular vesicles (EVs) from rheumatoid arthritis (RA) synovial fluid (SF) have been reported to stimulate the release of pro-inflammatory mediators in recipient cells. However, mechanistic details are unclear and contaminants in EV enrichments might have compromised previous observations. We recently developed a novel, size exclusion chromatography (SEC)-based method of EV isolation capable of high-quality enrichments from human synovial fluid. Here, we employed this method in combination with high resolution mass spectrometry to accurately characterise the SF EV proteome and investigate contributions of SF EVs to inflammatory processes in RA.

INSTRUMENT(S): impact II

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Rheumatoid Arthritis Disease Specific Synovial Fluid

DISEASE(S): Rheumatoid Arthritis

SUBMITTER: Laura Dagley  

LAB HEAD: Ken C. Pang

PROVIDER: PXD015145 | Pride | 2021-04-01

REPOSITORIES: Pride

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Dataset's files

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Action DRS
P3584_RAhigh_1_1_1_1_01_10071.d.zip Other
P3584_RAhigh_1_2_1_1_01_10072.d.zip Other
P3584_RAhigh_1_3_1_1_01_10073.d.zip Other
P3584_RAhigh_1_4_1_1_01_10074.d.zip Other
P3584_RAhigh_1_5_1_1_01_10075.d.zip Other
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Publications

Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid.

Foers Andrew D AD   Dagley Laura F LF   Chatfield Simon S   Webb Andrew I AI   Cheng Lesley L   Hill Andrew F AF   Wicks Ian P IP   Wicks Ian P IP   Pang Ken C KC  

Clinical & translational immunology 20201107 11

<h4>Objectives</h4>Extracellular vesicles (EVs) from rheumatoid arthritis (RA) synovial fluid (SF) have been reported to stimulate the release of pro-inflammatory mediators from recipient cells. We recently developed a size exclusion chromatography (SEC)-based method for EV isolation capable of high-quality enrichments from human SF. Here, we employed this method to accurately characterise the SF EV proteome and investigate potential contributions to inflammatory pathways in RA.<h4>Methods</h4>U  ...[more]

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