Dataset Information

Glioma Proteome explored by LC/MS/MS

ABSTRACT: Gliomas are the most frequent and aggressive malignancies of the central nervous system. Gliomas accumulate genetic alterations that cilminate in enhanced activity of receptor tyrosine kinasesand downstream mediators. The genetic alterations (gene amplification/loss) are well characterised, little information is available regarding the changes in the proteome of gliomas of different grades. Therefore, an unbiased quantitative proteomics approach of human gliomas has been undertaken. The proteomics results indicate various up- and downregulation of pathways, in particular downregulation of the endocytic machinery components involved in initiation, formation, and scission of endocytic carriers. Both clathrin-dependent and -independent endocytosis were changed. The reduction of endocytic machinery components causes increased receptor cell surface levels, a prominent phenotype of defective endocytosis. The impaired endocytosis creates a selective advantage in glioma tumour progression due to prolonged receptor tyrosine signalling from the cell surface.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Brain White Matter

SUBMITTER: Paul Jenö

LAB HEAD: Paul Jenoe

PROVIDER: PXD014606 | Pride | 2019-07-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170726_BTB066_Fr1_01.raw	Raw
	20170726_BTB066_Fr1_02.raw	Raw
	20170726_BTB066_Fr1_03.raw	Raw
	20170726_BTB066_Fr2_01.raw	Raw
	20170726_BTB066_Fr2_02.raw	Raw

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Publications

Quantitative proteomics reveals reduction of endocytic machinery components in gliomas.

Buser Dominik P DP Ritz Marie-Françoise MF Moes Suzette S Tostado Cristobal C Frank Stephan S Spiess Martin M Mariani Luigi L Jenö Paul P Boulay Jean-Louis JL Hutter Gregor G

EBioMedicine 20190719

<h4>Background</h4>Gliomas are the most frequent and aggressive malignancies of the central nervous system. Decades of molecular analyses have demonstrated that gliomas accumulate genetic alterations that culminate in enhanced activity of receptor tyrosine kinases and downstream mediators. While the genetic alterations, like gene amplification or loss, have been well characterized, little information exists about changes in the proteome of gliomas of different grades.<h4>Methods</h4>We performed ...[more]

PMID: 31331834

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