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ITRAQ-based phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication

ABSTRACT: Pseudorabies virus (PRV), the etiological agent of Aujeszky’s disease, is a swine pathogen of the alphaherpesvirinae subfamily resulting in devastating neurological and respiratory disorders in piglets, or abortion in pregnant sows.Here, we performed isobaric tags for relative and absolute quantitation (iTRAQ) quantitative phosphoproteomics on PRV-infected PK-15 cells, which identified 5723 phospho-peptides, corresponding to 2180 proteins, including 810 proteins showing significant changes in phosphorylation level during early PRV infection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Zheng-Fei Liu

LAB HEAD: Zheng-Fei Liu

PROVIDER: PXD014685 | Pride | 2022-10-13

REPOSITORIES: Pride

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			Action	DRS
	P17105.pep.xml	Pepxml
	P17105.prot.xml	Xml
	P17105.raw	Raw
	P17105_170324205849.raw	Raw
	P17105_170325020908-01.msf	Msf

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Publications

Phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication.

Jiang Fang-Fang FF Wang Ren-Qi RQ Guo Chao-Yue CY Zheng Ke K Liu Hai-Long HL Su Le L Xie Sheng-Song SS Chen Huan-Chun HC Liu Zheng-Fei ZF

Virologica Sinica 20220607 4

Pseudorabies virus (PRV), an etiological agent of pseudorabies in livestock, has negatively affected the porcine industry all over the world. Epithelial cells are reported as the first site of PRV infection. However, the role of host proteins and its related signaling pathways in PRV replication is largely unclear. In this study, we performed a quantitative phosphoproteomics screening on PRV-infected porcine kidney (PK-15) epithelial cells. Totally 5723 phosphopeptides, corresponding to 2180 pro ...[more]

PMID: 35688418

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Phosphoproteomic Landscape of Pseudorabies Virus Infection Reveals Multiple Potential Antiviral Targets

Project description:A quantitative phosphoproteome was conducted to explore the phosphorylation changes and host cellular responses upon PRV infection. Besides, the characteristics of the phosphorylated proteins were analyzed, including molecular distribution, percentage of the phosphorylated (STY) sites, and phosphorylated motifs. Additionally, the GO and KEGG analyses of the significantly changed phophoproteins were performed. Finally, DNMT1, HDAC6, EGFR, GRK2, VIM, and G3BP1 were chosen for analysis of their effect on PRV replication using an inhibitory-based experiment, and the results showed that all these proteins are necessary for efficient PRV replication.

2023-11-17 | PXD044682 | Pride

Porcine PARP11 is essential for the proliferation of pseudorabies Virus

Project description:PRV infection in swine can cause devastating disease and pose a potential threat to humans. Advancing the interplay between PRV and host is essential to elucidate the pathogenic mechanism of PRV and identify novel anti-PRV targets. In this study, we identified PARP11 as a host factor that can significantly affect PRV infection. Inhibition of PARP11 and knockout of PARP11 can significantly promoted PRV infection. Subsequently, we further found that PARP11 knockout upregulated the transcription of NXF1 and CRM1, resulting in enhanced transcription of viral genes. Furthermore, we also found that PARP11 knockout could activate the autophagy pathway and suppress the mTOR pathway during PRV infection. These findings could provide insight into the mechanism in which PARP11 participated during PRV infection and offer a potential target to develop anti-PRV therapies.

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Long Non-Coding RNA and mRNA profiling using High-throughput RNA sequencing in pseudorabies virus type II infected cells

Project description:Pseudorabies, an acute infectious disease caused by pseudorabies virus (PRV), has caused enormous economic losses to the breeding industry in many countries worldwide. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) may play important roles in the antiviral responses. However, little is known about the identification and functions of swine lncRNAs in cellular antiviral responses against PRV II. In this study, we detected the expression profiles of host lncRNAs and mRNAs from PRV-DX, a wild-type (WT) strain of PRV II, and its attenuated gE-TK- PRV-DX infected cells by high-throughput RNA sequencing. Finally, we identified differentially expressed (DE) 664 lncRNAs and DE 7,199 mRNAs from PRV-DX infected cells, and 654 DE lncRNAs and DE 7,149 mRNAs from gE-TK- PRV-DX infected cells when compared to MOCK infected cells, respectively, especially including 469 common DE lncRNAs and 5836 DE mRNAs. Besides 276 DE lncRNAs and 2,272 DE mRNAs between PRV-DX and gE-TK- PRV-DX infected cells were identified. The potential functions of the significant DE lncRNAs were involved in interleukin secretion, axon extension and metabolic process based on the Gene ontology and Kyoto Encyclopedia of Genes and Genomes databases. Taken together, results highlighted the potentials of lncRNA as targets for antiviral therapy and enriched the current knowledge of the mechanisms underlying the interaction between PRV II and its host cells.

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B27 inmunopeptidome in vaccinica infected cells

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The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation

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