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Arabidopsis thaliana leaf / E.coli / MEF proteomes label-free shotgun

ABSTRACT: Proteomic comparison of Arabidopsis thaliana leaf / E.coli / MEF proteomes generated by digestion with legumain, GluC and trypsin.

INSTRUMENT(S): maXis

ORGANISM(S): Escherichia Coli Arabidopsis Thaliana (mouse-ear Cress) Mus Musculus (mouse)

TISSUE(S): Cell Culture, Leaf

SUBMITTER: Fatih Demir

LAB HEAD: Pitter Florian Huesgen

PROVIDER: PXD014696 | Pride | 2020-01-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Specificity_Shotgun_MQ_txt.zip	Other
	Specificity_Shotgun_Raw_Data.zip	Other

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Publications

ExteNDing Proteome Coverage with Legumain as a Highly Specific Digestion Protease.

Soh Wai Tuck WT Demir Fatih F Dall Elfriede E Perrar Andreas A Dahms Sven O SO Kuppusamy Maithreyan M Brandstetter Hans H Huesgen Pitter F PF

Analytical chemistry 20200131 4

Bottom-up mass spectrometry-based proteomics utilizes proteolytic enzymes with well characterized specificities to generate peptides amenable for identification by high-throughput tandem mass spectrometry. Trypsin, which cuts specifically after the basic residues lysine and arginine, is the predominant enzyme used for proteome digestion, although proteases with alternative specificities are required to detect sequences that are not accessible after tryptic digest. Here, we show that the human cy ...[more]

PMID: 31951383

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Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)-msr(D) macrolide efflux system in Streptococcus pneumoniae.

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