Dataset Information

Interplay of redox modulators and carbohydrate metabolism enzymes in Dioscorea alata tuber degradation and formation.

ABSTRACT: Dioscorea tuber undergoes multiple morphological and bio-chemical changes during its 9 month growth period. A stage specific gel free analysis was done to understand the proteomic changes associated with tuber development and assign markers. On the basis of morphological traits the tuber life cycle was divided into four developmental stages namely; root initiation (S1), vegetative growth (S2), new tuber initiation (S3) and tuber maturation (S4) which was validated by principal component analysis (PCA). The first most comprehensive data set was generated by using the pooled genome information from Dioscorea + Solanum + Viridateplantae as reference set identifying 78.2% of the total 3,681 proteins. The over-representation analysis of proteins using PANTHER and KEGG MAPPER revealed both expected and novel biological processes relevant to each developmental stage. A high abundance of the enzymes of ascorbate-glutathione cycle, carbohydrate metabolism, Glycolysis, TCA cycle was detected during tuber degradation and formation. The Glycolytic and starch biosynthesis pathway were re-constructed using the information derived from the proteome data. Novel transcription factors (14) associated with oxidative stress tolerance were identified in D.alata proteome. In conclusion, the data set comprehensively describes the proteome of Dioscorea tuber and provided growth specific markers (APx, MDHAR, invertase for degradation and sucrose synthase for formation) that would pave the way to a systematic study of the tuber. The study provides information that may influence the direction of research for improving the productivity of this under-utilized crop.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Dioscorea Alata

TISSUE(S): Plant Cell, Tuber

SUBMITTER: Shruti Sharma

LAB HEAD: Renu Deswal

PROVIDER: PXD014734 | Pride | 2021-01-04

REPOSITORIES: Pride

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Publications

Dioscorea Alata Tuber Proteome Analysis Uncovers Differentially Regulated Growth-associated Pathways of Tuber Development.

Sharma Shruti S Deswal Renu R

Plant & cell physiology 20210301 1

During its life cycle, the Dioscorea tuber undergoes multiple morphological and biochemical changes. To gain a better understanding of the metabolic changes associated with tuber growth, a stage-specific gel-free proteome analysis of four distinct morphological stages namely germinating tuber (S1), degrading tuber (S2), new tuber formation (S3) and tuber maturation (S4) was done and validated by principal component analysis. A comprehensive data set identifying 78.2% of the total 3,681 proteins ...[more]

PMID: 33313836

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Project description:Helianthus tuberosus L., known as the Jerusalem Artichoke, is a hexaploid plant species, adapted to low nutrient soils, that accumulates high levels of inulin in its tubers. Inulin is a fructose-based polysaccharide used either as dietary fiber or for the production of bioethanol. Key enzymes involved in inulin biosynthesis are well known. However, the gene networks underpinning tuber development and inulin accumulation in H. tuberous remain elusive. To fill this gap, we selected 6,365 ESTs from a H. tuberosus library to set up a microarray platform and record their expression across three tuber developmental stages, when rhizomes start enlarging (T0), at maximum tuber elongation rate (T3) and at tuber physiological maturity (Tm), in “VR” and “K8-HS142”clones. The former was selected as an early tuberizing, the latter as a late-tuberizing clone. We quantified inulin and starch levels, and q-RT PCR confirmed the expression of key genes accounting for inulin biosynthesis. The microarray analysis revealed that the differences in morphological and physiological traits between tubers of the two clones are genetically determined since T0 and that is relatively low the number of differentially expressed ESTs across the stages shared between the clones (93). The expression of ESTs for sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan:fructan 1-fructosyltransferase (1-FFT), the two critical genes for fructans polymerization, resulted to be temporarily synchronized and mirror the progress of inulin accumulation and stretching. The expression of ESTs for starch biosynthesis was insignificant throughout the developmental stages of the clones in line with the negligible level of starch into their mature tubers, where inulin was the dominant polysaccharide. Overall, our study disclosed candidate genes underpinning the development and storage of carbohydrates in the tubers of two H. tuberosus clones. A model according to which the steady state levels of 1-SST and 1-FFT transcripts are developmentally controlled and might represent a limiting factor for inulin accumulation has been provided. Our finding may have significant repercussions for breeding clones with improved levels of inulin for food and chemical industry.

Dataset Information

Interplay of redox modulators and carbohydrate metabolism enzymes in Dioscorea alata tuber degradation and formation.

Publications

Dioscorea Alata Tuber Proteome Analysis Uncovers Differentially Regulated Growth-associated Pathways of Tuber Development.

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