Dataset Information

Cytosolic and plasma membrane proteome analysis of rice leaves in response to MSP1 treatment

ABSTRACT: MSP1 is a Magnaporthe oryzae secreted protein that elicits defense responses in rice. However, the molecular mechanism of MSP1 action is largely elusive. Here, we employed a TMT-based quantitative proteomic analysis of cytoplasmic as well as plasma membrane proteins to decipher the MSP1 induced signalling in rice. This approach led to the identification of 6691 proteins of which 3049 were identified in the plasma membrane (PM) while 3642 were identified in the cytoplasmic fraction. A parallel phosphoproteome analysis led to the identification of 1906 phosphopeptides and integration of proteome and phosphoproteome data showed activation of proteins related to the proteolysis, jasmonic acid biosynthesis, redox metabolism and MAP kinase signaling pathways in response to MSP1 treatment. Further, MSP1 induced phosphorylation of some of the key proteins including RBOHB, MEKK1, MPK3/6, CDPK and CaM suggest activation of PAMP-triggered immunity (PTI) in response to MSP1 treatment. In essence, our results further support the functioning of MSP1 as a PAMP and provide an overview of the MSP1 induced signaling in rice leaves.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Leaf

SUBMITTER: Sun Tae Kim

LAB HEAD: Sun Tae Kim

PROVIDER: PXD014758 | Pride | 2019-08-30

REPOSITORIES: Pride

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Publications

Identification of Msp1-Induced Signaling Components in Rice Leaves by Integrated Proteomic and Phosphoproteomic Analysis.

Gupta Ravi R Min Cheol Woo CW Kim Yu-Jin YJ Kim Sun Tae ST

International journal of molecular sciences 20190824 17

MSP1 is a <i>Magnaporthe oryzae</i> secreted protein that elicits defense responses in rice. However, the molecular mechanism of MSP1 action is largely elusive. Moreover, it is yet to be established whether MSP1 functions as a pathogen-associated molecular pattern (PAMP) or an effector. Here, we employed a TMT-based quantitative proteomic analysis of cytosolic as well as plasma membrane proteins to decipher the MSP1 induced signaling in rice. This approach led to the identification of 6691 prote ...[more]

PMID: 31450622

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