Proteomics

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Surfaceome dynamics and surface trafficking during neuronal development and synaptic plasticity

ABSTRACT: Surface proteins are of fundamental importance for formation of synaptic connections and activity-dependent plasticity. Here, we used a spatiotemporally resolved cell-surface proteotype analysis to characterize the neuronal surface-exposed proteome, or surfaceome, during neuronal development and synapse formation in primary neuronal cultures. We established a map of the neuronal surfaceome, which includes about 1,000 surface proteins, and analyzed the dynamic remodeling of the quantitative surfaceome during development. We identified time-resolved surface-abundance profile clusters that correspond to distinct stages of neuronal development. We discovered that surface abundance changes can correlate with or be uncoupled from the total cellular abundance. Finally, we observed system-wide surfaceome modulation in response to homeostatic synaptic scaling and exocytosis of diverse cargo during long-term potentiation.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Marc van Oostrum  

LAB HEAD: Bernd Wollscheid

PROVIDER: PXD014790 | Pride | 2020-10-05

REPOSITORIES: Pride

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Publications

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity.

van Oostrum Marc M   Campbell Benjamin B   Seng Charlotte C   Müller Maik M   Tom Dieck Susanne S   Hammer Jacqueline J   Pedrioli Patrick G A PGA   Földy Csaba C   Tyagarajan Shiva K SK   Wollscheid Bernd B  

Nature communications 20201005 1

Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While brain transcriptome, connectome and global proteome maps are being generated, system-wide analysis of temporal protein dynamics at the subcellular level are currently lacking. Here, we perform a temporally-resolved surfaceome analysis of primary neuron cultures and reveal dynamic surface protein clusters that reflect the functional requirements during distinct stages of neuronal development  ...[more]

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