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Interactome of the TCF3-HLF fusion protein

ABSTRACT: Oncogenic chimeric transcription factors are central drivers in cancer. To understand how the TCF3-HLF fusion protein rewires the transcriptional landscape in t(17;19) positive leukemia, functional genetic and proteomic experiments were conducted. In this dataset, the protein-protein interactions of the endogenous TCF3-HLF complex were characterized by AP-MS.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fabian Frommelt

LAB HEAD: Jean-Pierre Bourquin

PROVIDER: PXD014992 | Pride | 2020-08-05

REPOSITORIES: Pride

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			Action	DRS
	20171013_02_HAL01_4.raw	Raw
	20171013_03_8-17_7.raw	Raw
	20171013_05_HAL01_2.raw	Raw
	20171013_12_8-17_8.raw	Raw
	20171013_14_HAL01_3.raw	Raw

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Publications

The Leukemogenic TCF3-HLF Complex Rewires Enhancers Driving Cellular Identity and Self-Renewal Conferring EP300 Vulnerability.

Huang Yun Y Mouttet Brice B Warnatz Hans-Jörg HJ Risch Thomas T Rietmann Fabian F Frommelt Fabian F Ngo Quy A QA Dobay Maria Pamela MP Marovca Blerim B Jenni Silvia S Tsai Yi-Chien YC Matzk Sören S Amstislavskiy Vyacheslav V Schrappe Martin M Stanulla Martin M Gstaiger Matthias M Bornhauser Beat B Yaspo Marie-Laure ML Bourquin Jean-Pierre JP

Cancer cell 20191114 6

The chimeric transcription factor TCF3-HLF defines an incurable acute lymphoblastic leukemia subtype. Here we decipher the regulome of endogenous TCF3-HLF and dissect its essential transcriptional components and targets by functional genomics. We demonstrate that TCF3-HLF recruits HLF binding sites at hematopoietic stem cell/myeloid lineage associated (super-) enhancers to drive lineage identity and self-renewal. Among direct targets, hijacking an HLF binding site in a MYC enhancer cluster by TC ...[more]

PMID: 31735627

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