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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human BM-MSC expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability

ABSTRACT: hBM-MSC were expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability;ÃÂµA analysis were performed p2/p5 (n=10)and p5/p10 (n=5) to examine if the genetic background of hBM-MSC is changeing during their high-grade expansion;

ORGANISM(S): Homo sapiens

SUBMITTER: paar Christine Paar

PROVIDER: E-MARS-6 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation.

Kulterer Birgit B Friedl Gerald G Jandrositz Anita A Sanchez-Cabo Fatima F Prokesch Andreas A Paar Christine C Scheideler Marcel M Windhager Reinhard R Preisegger Karl-Heinz KH Trajanoski Zlatko Z

BMC genomics 20070312

<h4>Background</h4>Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influ ...[more]

PMID: 17352823

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2007-06-09 | E-MARS-4 | biostudies-arrayexpress

Transcription profiling of human BM-MSC expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability

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Project description:Experiments were designed to compare white adipose tissue (WAT) or brown adipose tissue (BAT) -in male and female mice- between Bscl2 knock-out mice and their wild-type control mice. RNA was pooled to obtain 2x 8µg per tissue source and subjected to dye-swap hybridization.

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Transcription profiling of dfferentiation of human bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction

Project description:Differentiation of bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction; reference d-1 (one day before induction; preconfluent 70-80%)

2007-06-09 | E-MARS-3 | biostudies-arrayexpress

Transcription profiling of wild type and A-C/EBP transgenic mouse embryonic fibroblasts during adipogenesis to identify C/ebp alpha target genes

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Transcription profiling of human hMADS cells during adipocyte and osteoblast differentiation

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2008-08-08 | E-MARS-10 | biostudies-arrayexpress

Transcription profiling of baboon organs to identify late host responses to Gram positive (S. pyogenes) or Gram negative (E. coli) induced sepsis

Project description:The aim of the project is to identify the late (24h) host response related to Gram positive (S. pyogenes) or Gram negative (E. coli) induced sepsis in a controlled sepsis model 24 hours a. Therefore a well defined baboon sepsis model, established at the Ludwig Boltzmann Institute for experimental and clinical traumatology of Vienna, is used. The overall goal of the project is to define differentially expressed or processed genes which give rise to diagnostic and therapeutic targets and consequently better monitoring and earlier onset of therapy.

2010-04-27 | E-MARS-15 | biostudies-arrayexpress

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2010-04-27 | E-MARS-14 | biostudies-arrayexpress

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2010-03-26 | E-MARS-17 | biostudies-arrayexpress

Transcription profiling by array of hMADS cells transfected with miR-26a

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