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Human glioma cell-line proteomics upon treatment with different drugs

ABSTRACT: Increasing evidence suggests that induction of lethal autophagy carries potential significance for the treatment of glioblastoma (GBM). In continuation of our previous work, we investigated if autophagy-inducing compunds can trigger ATG-dependent cell death in human MZ-54 GBM cells and which pathways may be affected using TMT10-based quantitative proteomics as one of the tools.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Georg Tascher

LAB HEAD: Christian Münch

PROVIDER: PXD015074 | Pride | 2021-02-26

REPOSITORIES: Pride

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	20180619_GT_Nina_WCP_F1.raw	Raw
	20180619_GT_Nina_WCP_F2.raw	Raw
	20180619_GT_Nina_WCP_F3.raw	Raw
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Publications

Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death.

Meyer Nina N Henkel Lisa L Linder Benedikt B Zielke Svenja S Tascher Georg G Trautmann Sandra S Geisslinger Gerd G Münch Christian C Fulda Simone S Tegeder Irmgard I Kögel Donat D

Autophagy 20210119 11

Increasing evidence suggests that induction of lethal macroautophagy/autophagy carries potential significance for the treatment of glioblastoma (GBM). In continuation of previous work, we demonstrate that pimozide and loperamide trigger an ATG5- and ATG7 (autophagy related 5 and 7)-dependent type of cell death that is significantly reduced with cathepsin inhibitors and the lipid reactive oxygen species (ROS) scavenger α-tocopherol in MZ-54 GBM cells. Global proteomic analysis after treatment wit ...[more]

PMID: 33461384

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Studies of autophagy pathway during submergence in Arabidopsis thaliana

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Proteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells

Project description:Previously (Ly et al. 2014), we analyzed protein abundance changes across a â€˜minimally perturbedâ€™ cell cycle in human cells (NB4), using centrifugal elutriation to differentially enrich distinct cell cycle phases. Here, we compare data from elutriated cells with NB4 cells arrested at comparable phases using serum starvation, hydroxyurea, or RO-3306. While elutriated and arrested cells have similar patterns of DNA content and cyclin expression, a large fraction of the proteome changes detected in arrested cells are found to reflect arrest-specific responses (i.e., starvation, DNA damage, CDK1 inhibition), rather than physiological cell cycle regulation. For example, we show most cells arrested in G2 by CDK1 inhibition express abnormally high levels of replication and origin licensing factors, and are likely poised for genome re-replication. The protein data are available in the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/), an online, searchable resource.

2015-07-23 | PXD001610 | Pride

Proteomic characterization of plasma-derived exosomes from P. vivax infected FRG huHep mice

Project description:This project focused in the proteomic characterization of plasma-derived exosomes from Plasmodium vivax infected FRG huHep mice, a suitable in vivo model for the development of pre-erythrocytic stages of this parasite. P. vivax is responsible for the vast majority of Malaria cases outside Africa. This parasite evolved a latent liver stage form called hypnozoite that cannot be detected using the current diagnostic methods. This represent a major limitation to the Malaria elimination goal. Exosomes are extracellular vesicles involved in intercellular communication and in a large variety of physiological functions. Recently, this vesicles have been recognized for the immense potential to be used as biomarkers in the context of non-invasive diagnostic approaches from liquid biopsies. The exploration of the protein content of exosomes secreted into the bloodstream of P. vivax infected FRG huHep mice represents an interesting approach to tackle the big challenge of identifying a hypnozoite biomarker which in the future could help to identify hypnozoites carriers in Malaria vivax relapsing patients.

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Transcriptional regulation by Pho23 modulates the frequency of autophagosome formation.

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The identification of Dwg downstream targets using ChIP-seq

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Translational control of autophagy by Orb in the Drosophila germline [mature]

Project description:Drosophila Orb, the homologue of vertebrate CPEB is a key translational regulator involved in oocyte polarity and maturation through poly(A) tail elongation of specific mRNAs. orb has also an essential function during early oogenesis which has not been addressed at the molecular level. Here, we show that orb prevents cell death during early oogenesis, thus allowing oogenesis to progress. It does so through the repression of autophagy, by directly repressing, together with the CCR4 deadenylase, the translation of Autophagy-specific gene 12 (Atg12) mRNA. Autophagy and cell death observed in orb mutant ovaries are reduced by decreasing Atg12 or other Atg mRNA levels. These results reveal a role of Orb in translational repression and identify autophagy as an essential pathway regulated by Orb during early oogenesis. Importantly, they also establish translational regulation as a major mode of control of autophagy, a key process in cell homeostasis in response to environmental cues. Orb RIP-chip vs mock IP on ovaries from mature 3-5 day old females.

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Translational control of autophagy by Orb in the Drosophila germline [early]

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Autophagy and alternative NF-kappaB inhibition by RNAi against RELB, ATG5 or ULK1 in A549 human cancer cells

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Expression data from PDE1C knockdown glioblastoma short-term cultures

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Transcriptional regulation by Pho23 modulates the frequency of autophagosome formation.

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