Dataset Information

GUN1 controls the accumulation of NEP-dependent transcripts in Arabidopsis cotyledons

ABSTRACT: GUN1 proteins controls protein homeostasis in chloroplast development in cotyledons of the model plant Arabidopsis thaliana, via coordination of nuclear encoded polymerase (NEP)-dependent chloroplast genes expression with plastid encoded polymerase (PEP)-dependent chloroplast genes expression. Lack of GUN1 leads to development of abnormal plastids and, consequently, accumulation of nuclear-encoded chloroplast-targeted (NECT) proteins which in many cases have been found still in their precursor form. Data dependent acquisition (DDA) mass spectrometry analysis of cotyledons soluble fractions, as well as targeted proteomics analysis of specific FtsH protease forms recognized by FtsH antibodies in western blotting, have been performed to identify peptides from the chloroplast transit peptide (cTP) of NECT in cotyledons extracts of wild type and GUN1 knocked-out mutant plants. The aim was to compare the number of cTPs found in 6 days after sowing (DAS) seedlings grown on plates with or without the inhibitor of plastid translation lincomycin.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Cotyledon

SUBMITTER: Andrea Trotta

LAB HEAD: Eva-Mari Aro

PROVIDER: PXD015078 | Pride | 2019-11-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Col-0_biol1_Supernatant.msf	Msf
	Col-0_biol1_Supernatant.mzML	Mzml
	Col-0_biol1_Supernatant.mzid.gz	Mzid
	Col-0_biol1_Supernatant.pride.mgf.gz	Mgf
	Col-0_biol1_Supernatant.raw	Raw

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Publications

GUN1 influences the accumulation of NEP-dependent transcripts and chloroplast protein import in Arabidopsis cotyledons upon perturbation of chloroplast protein homeostasis.

Tadini Luca L Peracchio Carlotta C Trotta Andrea A Colombo Monica M Mancini Ilaria I Jeran Nicolaj N Costa Alex A Faoro Franco F Marsoni Milena M Vannini Candida C Aro Eva-Mari EM Pesaresi Paolo P

The Plant journal : for cell and molecular biology 20191213 5

Correct chloroplast development and function require co-ordinated expression of chloroplast and nuclear genes. This is achieved through chloroplast signals that modulate nuclear gene expression in accordance with the chloroplast's needs. Genetic evidence indicates that GUN1, a chloroplast-localized pentatricopeptide repeat (PPR) protein with a C-terminal Small MutS-Related (SMR) domain, is involved in integrating multiple developmental and stress-related signals in both young seedlings and adult ...[more]

PMID: 31648387

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