Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mass spectrometry detection of photodamage in human extracellular matrix assemblies

ABSTRACT: Photoageing in skin is commonly recognised by architectural remodelling of dermal extracellular matrix components. Mass spectrometry was previously used to identify tissue-specific patterns of fibrillin-1 and collagen VI peptide spectrum matches (PXD008450). This study aimed to determine if the same mass spectrometry-based approach could detect peptide spectrum match patterns and significantly differences in relative abundance of peptide sequences characteristic of damage following exposure to UVR of co-purified suspensions of fibrillin and collagen VI microfibrils. Human dermal fibroblast-derived suspensions of microfibrils were irradiated with either broadband UVB or solar simulated radiation (SSR). UVR-induced molecular damage was characterised by proteolytic peptide generation with elastase followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). This allowed the molecular scale identification of UV-induced structural changes within two skin matrix assemblies. The proteomic approaches used have the potential to facilitate the rapid, protein-specific identification of differential molecular fingerprints of damage in key extracellular matrix proteins.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Alexander Eckersley

LAB HEAD: Michael Sherratt

PROVIDER: PXD015149 | Pride | 2020-01-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20171206_AE_E_A01.raw	Raw
	20171206_AE_E_A01.raw (Elastase_M79_Control).mzid_20171206_AE_E_A01.raw_(Elastase_M79_Control).pride.mgf.gz	Mzid
	20171206_AE_E_A01.rawElastase_M79_Control.mzid.gz	Mzid
	20171206_AE_E_A01.rawElastase_M79_Control.mzid_20171206_AE_E_A01.raw_Elastase_M79_Control.MGF	Mzid
	20171206_AE_E_A02.raw	Raw

Items per page:

1 - 5 of 36

Similar Datasets

The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana

Project description:We present a functional characterisation of two members of the IDA-LIKE (IDL) peptide family in Arabidopsis thaliana, IDL6 and IDL7. They are processed both C- and N-terminally to produce active peptides. Structure analyses of synthesized IDL6 and IDL7 peptides indicate that they lack secondary structure elements. Localisation studies suggest that the peptides require a signal peptide and C-terminally processing to be correctly transported out of the cell. Treatment of plants with synthetic IDL6 and IDL7 peptides resulted in down-regulation of a broad range of stress-responsive genes, including early stress-responsive transcripts, dominated by a large group of ZINC FINGER PROTEINS (ZFPs), WRKYs and genes encoding calcium-dependent proteins. idl6 and idl7 mutants were more tolerant to salt, whereas the respective overexpression lines displayed increased sensitivity to both salt and oxidative stress. Taken together, our results suggest that the putative peptide ligands IDL6 and IDL7 act as suppressors of abiotic stress responses in Arabidopsis. Two weeks old seedlings were treated either with 100 nM IDL6 or IDL7 peptide (treated) or 100 nM mock peptide (control) and whole rosettes were harvested 2 hours after treatment. 4 biological replicaes per treatment. Two color microarray. Biological replicas are dye-swapped between slides.

2016-03-31 | E-GEOD-77467 | biostudies-arrayexpress

Screening of rheumatoid arthritis patients sera against citrullinated fibrinogen peptides using ImmunoCAP ISAC system

Project description:Citrullinated and unmodified peptides (>95% purity, ProImmune AB) were immobilized onto a chemically modified glass slide, sera from RA patients and healthy controls were applied into the reactions sites and fluorescence intensity after incubation with anti-human IgG antibody was acquired in a laser scanner. Final results for each citrullinated peptide were calculated by subtracting the intensity values of corresponding arginine containing control peptide from citrullinated peptide for all RA patients and controls.

2015-10-25 | E-MTAB-3606 | biostudies-arrayexpress

Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts [II]

Project description:Skin damage from solar ultraviolet radiation (UVR) accumulates in the dermal extracellular matrix (ECM) and contributes to photoaging. Following UVR exposure, matrix metalloproteinases (MMPs) are secreted by dermal fibroblasts to repair and remodel the ECM. Molecular signaling pathways delineating the induction of MMPs are currently well-defined; however, the effects of UV exposure on epigenetic mechanisms of MMP induction are not as well understood. An epigenetic mechanism would further describe how MMP genes are regulated in response to UV. In this study, we examined solar simulated UVR (ssUVR)-induced gene expression changes and alterations to histone methylation in the promoters of MMP1 and MMP3 in primary human dermal fibroblasts (HDF). This set of gene expression data was generated to identify photoaging related genes (including MMP) that were impacted by ssUVR exposure in our system. Primary neonatal human dermal fibroblasts (HDF) were irradiated a single time with 12 J/cm2 ssUVR. The sham treatments are negative controls (0 J/cm2 ssUVR). The cells were collected for gene expression analysis 1 day after exposure, and then 5 days after exposure. Affymetrix GeneChip Human Exon 1.0 ST arrays were used to characterize gene expression pattern alterations in response to ssUVR.

2014-08-27 | E-GEOD-60795 | biostudies-arrayexpress

Tissue Microfibril Comparison - Structural and compositional diversity of fibrillin microfibrils in human tissues

Project description:Fibrillin microfibrils are large supramolecular ECM assemblies which play different functional roles in different tissues. We aim to compare the ultrastructure and biological composition of fibrillin microfibrils purified from human eye, skin and cultured dermal fibroblasts. This will allow us to understand whether these their structure and compositions have evolved to suit the functions they play in different tissues.

2018-02-16 | PXD008450 | Pride

Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts [I]

Project description:Skin damage from solar ultraviolet radiation (UVR) accumulates in the dermal extracellular matrix (ECM) and contributes to photoaging. Following UVR exposure, matrix metalloproteinases (MMPs) are secreted by dermal fibroblasts to repair and remodel the ECM. Molecular signaling pathways delineating the induction of MMPs are currently well-defined; however, the effects of UV exposure on epigenetic mechanisms of MMP induction are not as well understood. An epigenetic mechanism would further describe how MMP genes are regulated in response to UV. In this study, we examined solar simulated UVR (ssUVR)-induced gene expression changes and alterations to histone methylation in the promoters of MMP1 and MMP3 in primary human dermal fibroblasts (HDF). This set of gene expression data was generated to identify photoaging related genes (including MMP) that were impacted by ssUVR exposure in our system. Primary neonatal human dermal fibroblasts (HDF) were irradiated a single time with 0, 4, or 12 J/cm2 ssUVR. The sham treatments are negative controls (0 J/cm2 ssUVR). The cells were collected for gene expression analysis 24 hours after exposure. Affymetrix GeneChip Human Exon 1.0 ST arrays were used to characterize gene expression pattern alterations in response to ssUVR.

2014-08-27 | E-GEOD-60794 | biostudies-arrayexpress

Transcriptomic responses in Arabidopsis thaliana to PIPL3 peptide treatment

Project description:In order to investigate possible roles of IDL and PIP/PIPL peptides, the transcriptomic response of Arabidopsis seedlings to treatment with PIPL3 peptide was analysed. PIPL3 (At4g37295) was chosen, as no functional data was available for this peptide; furthermore, PIPL3 was expressed in leaf tissue during seedling stages. Transcriptomic responses to 3 hours PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification. Two weeks old seedlings were treated either with 100 nM PIPL3 peptide (treated) or 100 nM mock peptide (control) and whole rosettes were harvested 3 hours after treatment. 4 biological replicates per treatment.

2015-07-10 | E-GEOD-66201 | biostudies-arrayexpress

XPC_UV

Project description:xpc-1 mutant animals were treated with 60mJ/cm2 UVB irradiation or mock treated to derive gene expression response to UVB induced DNA damage

2013-08-27 | E-MTAB-1689 | biostudies-arrayexpress

Single-cell RNA-seq of mouse epidermal or dermal samples

Project description:To identify differences between keratinocytes (w/o Pp6) and dermal dendritic cells, epidermal or dermal cell suspensions from C57BL/6 mice or K5. Pp6fl/fl mice were subject to single-cell RNA-seq.

2020-05-13 | GSE150361 | GEO

Single-cell RNA-seq of mouse epidermal or dermal samples in mouse psoriasiform skin

Project description:Epidermal or dermal cell suspensions from imiquimod-induced C57BL/6 mice were subject to single-cell RNA-seq.

2022-06-14 | GSE205790 | GEO

PAO1-1037

Project description:Gene expression in biofilms of PAO1 with and without the presence of 20 ug/ml peptide 1037 was analysed using microarrays.

2012-04-19 | E-MTAB-962 | biostudies-arrayexpress