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Proteomic Analysis of Measles Virus Protein C-Host Interactions in an Infectious Context Using Recombinant Viruses

ABSTRACT: Viruses manipulate central machineries of host cells to their advantage. They prevent host cell antiviral responses in order to create a favourable environment for their survival and propagation. Measles virus (MV) encodes two non-structural proteins MV-V and MV-C, proposed to counteract the host interferon response and to regulate cell death pathways in various functional assays. Several molecular mechanisms underlining MV-V regulation of innate immunity and cell death responses have been proposed, whereas MV-C host protein partners are less studied. We suggest that some cellular factors that are controlled by MV-C protein during viral replication could be components of innate immunity and the cell death pathways. In order to determine which host factors are hijacked by MV-C, we captured both direct and indirect host protein partners of MV-C protein. For this we used a strategy based on recombinant viruses expressing tagged viral proteins followed by affinity purification and a bottom-up mass spectrometry analysis. A list of host proteins specifically interacting with MV-C protein in different cell lines was identified.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human) Viruses

TISSUE(S): Epithelial Cell, Cell Culture, Embryonic Stem Cell

SUBMITTER: Thibaut Douché

LAB HEAD: Frédéric TANGY

PROVIDER: PXD015316 | Pride | 2021-01-19

REPOSITORIES: Pride

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			Action	DRS
	20160412_Q2_TD_ColQ2-12_Komarova_AM10_CH_278.raw	Raw
	20160412_Q2_TD_ColQ2-12_Komarova_AM10_Cter_272.raw	Raw
	20160412_Q2_TD_ColQ2-12_Komarova_AM10_Nter_275.raw	Raw
	20160412_Q2_TD_ColQ2-12_Komarova_AM11_CH_296.raw	Raw
	20160412_Q2_TD_ColQ2-12_Komarova_AM11_Cter_289.raw	Raw

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Dataset Information

Proteomic Analysis of Measles Virus Protein C-Host Interactions in an Infectious Context Using Recombinant Viruses

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