Dataset Information

PTPRR interactome detection using PUP-IT (pupylation-based interaction tagging) Mass Spectrometry

ABSTRACT: To further stably express PTPRR (WT)-PafA or PTPRR (DA)-PafA in iPUP OVCAR5 cell, we subcloned PTPRR-WT or DA, respectively, into the PafA-IRES-EGFP plasmid. Each plasmid was packed into a lentivirus and then transduced into iPUP OVACR5 cells for 48 h. GFP-positive cells were sorted by flow cytometry. The expression of PTPRR (WT)-PafA and PTPRR (DA)-PafA was confirmed by western blotting analysis. PTPRR (WT)-PafA or PTPRR (DA)-PafA expressed iPUP OVCAR5 cells were grown to a cell density of about 75% on 10 cm dishes. We followed the protocol established previous. To prepare PUP-IT samples for mass spectrometry analysis, including doxycycline induction, biotin labeling, cell lysis, streptavidin magnetic beads pull-down, trypsin digestion, and peptide cleaning.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ovarian Serous Adenocarcinoma Cell Line, Germinal Epithelium Of Ovary

DISEASE(S): Ovarian Carcinoma

SUBMITTER: Yuetong Wang

LAB HEAD: Gaofeng Fan

PROVIDER: PXD015352 | Pride | 2019-10-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ZhangJL_20180630_3ul_50cm_60min_DA-1.raw	Raw
	ZhangJL_20180630_3ul_50cm_60min_DA-2.raw	Raw
	ZhangJL_20180630_3ul_50cm_60min_DA-3.raw	Raw
	ZhangJL_20180630_3ul_50cm_60min_WT-1.raw	Raw
	ZhangJL_20180630_3ul_50cm_60min_WT-2.raw	Raw

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Publications

Protein tyrosine phosphatase receptor type R (PTPRR) antagonizes the Wnt signaling pathway in ovarian cancer by dephosphorylating and inactivating β-catenin.

Wang Yuetong Y Cao Jian J Liu Weiwei W Zhang Jiali J Wang Zuo Z Zhang Yiqun Y Hou Linjun L Chen Shengmiao S Hao Piliang P Zhang Liye L Zhuang Min M Yu Yang Y Li Dake D Fan Gaofeng G

The Journal of biological chemistry 20191025 48

Despite a lack of mutations, accumulating evidence supports an important role for the Wnt/β-catenin pathway in ovarian tumorigenesis. However, the molecular mechanism that contributes to the aberrant activation of the Wnt signaling cascade in ovarian cancer has not been fully elucidated. Here, we found that protein tyrosine phosphatase receptor type R (PTPRR) suppressed the activation of the Wnt/β-catenin pathway in ovarian cancer. We performed an shRNA-based biochemical screen, which identified ...[more]

PMID: 31653698

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