Dataset Information

Quantitative Proteomics Analysis of Membrane Glucocorticoid Receptor Activation in Rainbow Trout Skeletal Muscle

ABSTRACT: In the present work, we evaluated the effects of membrane-initiated cortisol actions in vivo in the proteome of rainbow trout (Oncorhynchus mykiss) skeletal muscle. Quantitative iTRAQ analyses were performed to examine proteomic changes in rainbow trout stimulated with physiological concentrations of cortisol and cortisol-BSA. A total of 873 proteins were identified, among which 38 proteins were commonly and differentially expressed under both conditions. Functional clustering analysis revealed an upregulation of proteins associated with mitochondria, metal-binding and secreted proteins.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Whole Body

SUBMITTER: Juan Valdes

LAB HEAD: Juan Valdes

PROVIDER: PXD015383 | Pride | 2019-10-30

REPOSITORIES: Pride

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Quantitative proteomics analysis of membrane glucocorticoid receptor activation in rainbow trout skeletal muscle.

Aedo Jorge E JE Fuentes-Valenzuela Marcia M Molina Alfredo A Valdés Juan A JA

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20191019

Cortisol modulates energy metabolism promoting the mobilization of glucose and increasing proteolysis to overcome stressful situations in teleost. The cortisol metabolic effects are attributed to genomic mechanisms that involve the interaction of cortisol with its glucocorticoid intracellular receptor. Furthermore, cortisol can also interact with plasma membrane glucocorticoid receptors activating a rapid nongenomic signaling; however, its contribution during the early acute phase stress respons ...[more]

PMID: 31655326

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