Dataset Information

TNP1 and TNP2 mRNA binding proteins from mouse testes

ABSTRACT: We hypothesized that specific proteins associate with Tnp mRNAs to suppress or stimulate their translation. We took a proteomics approach and developed a method to isolate endogenous mRNA species and their associated proteins. We employed biotinylated DNA oligonucleotides and avidin resin to capture endogenous Tnp mRNAs and associated molecules from testis extract, and we specifically eluted the complexes with non-biotinylated oligonucleotides. We then used mass spectrometry to identify proteins that were associated with the Tnp1 and Tnp2 mRNAs but not Actin mRNAs. We hypothesize that the proteins that are specifically enriched by Tnp1 and Tnp2 mRNA pulldowns relative to Actin mRNA pulldown may function to regulate these transcripts.

INSTRUMENT(S): 6340 Ion Trap LC/MS

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Sperm, Stem Cell

DISEASE(S): Disease Free

SUBMITTER: Jason Williams

LAB HEAD: Jason G. Williams

PROVIDER: PXD015384 | Pride | 2019-10-04

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	DataforPhillipsetal2019.zip	Other
	Phillips_etal_TNP1_TNP2_pulldown_search_results.xlsx	Xlsx

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Publications

Mass spectrometric identification of candidate RNA-binding proteins associated with Transition Nuclear Protein mRNA in the mouse testis.

Phillips Bart T BT Williams Jason G JG Atchley Dustin T DT Xu Xiaojiang X Li Jian-Liang JL Adams Andrea L AL Johnson Katina L KL Hall Traci M Tanaka TMT

Scientific reports 20190920 1

Spermatogenesis is a differentiation process that requires dramatic changes to DNA architecture, a process governed in part by Transition Nuclear Proteins 1 and 2 (TNP1 and TNP2). Translation of Tnp1 and Tnp2 mRNAs is temporally disengaged from their transcription. We hypothesized that RNA regulatory proteins associate specifically with Tnp mRNAs to control the delayed timing of their translation. To identify potential regulatory proteins, we isolated endogenous mRNA/protein complexes from testi ...[more]

PMID: 31541158

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