Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Analysis of transcriptome changes in HeLa cells after knock-down of variant U1.8 snRNA


ABSTRACT: U1 small nuclear (sn)RNA, required for splicing of pre-mRNA, is encoded by genes on chromosome 1p36. Imperfect copies of these ‘true’ (t)U1 snRNA genes, located on chromosome 1q12-21, were thought to be pseudogenes. However, many of these ‘variant’ (v)U1 snRNA genes produce fully-processed transcripts that are packaged into potentially functional particles. Using antisense oligonucleotides, we have achieved functional knockdown of a specific vU1 snRNA in HeLa cells and identified over 400 transcriptome changes following interrogation of the Affymetrix Human Exon ST 1.0 array. Total RNA from 4 biological repeats of vU1.8 snRNA and control knock-down were analysed using Affymetrix Human Exon ST 1.0 Array.

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Dienstbier 

PROVIDER: E-GEOD-37623 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-10-01 | GSE37623 | GEO
2009-02-21 | E-GEOD-14434 | biostudies-arrayexpress
2020-01-01 | GSE124951 | GEO
2023-12-04 | PXD045484 | Pride
2023-03-03 | PXD016607 | Pride
2015-02-10 | E-GEOD-64078 | biostudies-arrayexpress
2023-09-07 | PXD029392 | Pride
2011-10-19 | E-GEOD-33069 | biostudies-arrayexpress
2019-11-27 | E-MTAB-7635 | biostudies-arrayexpress
2023-12-27 | PXD044643 | Pride