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Nitrate Starvation time course membrane protein phosphoproteomics

ABSTRACT: Nitrate nutrtition was withdrawn from Arabiodpsis plants to study early responses upon nitrate depletion. Plants were grown under full nutrition (3mM nitrate) for three weeks and then transferred to nutrient solution without nitrate for 15 minutes and 3 hours.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Root

SUBMITTER: Waltraud Schulze

LAB HEAD: Waltraud Schulze

PROVIDER: PXD015390 | Pride | 2024-05-21

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MaxQuant_Output.zip	Other
	experimentalDesign_NO3_starvation.txt	Txt
	wt_no_0_1.raw	Raw
	wt_no_0_1r.raw	Raw
	wt_no_0_2.raw	Raw

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1 - 5 of 14

Publications

Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis.

Li Zhi Z Na Wu Xu X Jacquot Aurore A Chaput Valentin V Adamo Mattia M Neuhäuser Benjamin B Straub Tatsiana T Lejay Laurence L Schulze Waltraud X WX

Journal of experimental botany 20240301 7

NRT2.1, the major high affinity nitrate transporter in roots, can be phosphorylated at five different sites within the N- and the C-terminus. Here, we characterized the functional relationship of two N-terminal phosphorylation sites, S21 and S28, in Arabidopsis. Based on a site-specific correlation network, we identified a receptor kinase (HPCAL1, AT5G49770), phosphorylating NRT2.1 at S21 and resulting in active nitrate uptake. HPCAL1 itself was regulated by phosphorylation at S839 and S870 with ...[more]

PMID: 38066636

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