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Perception of the peptide hormone Systemin

ABSTRACT: Cell suspension cultures of Solanum peruvianum were subjected to stimulation with 10nM systemin, the inactive systemin analog10nM a17, or injected with equal volume of water. For each treatment, a batch of cells was harvested after 2 minutes, 5 minutes, 15 minutes and 45 minutes. Untreated cells were harvested as controls. All treatments and time points were sampled as three independent replicates using independent sets of cells. Microsomal membranes were isolated from each sample, digested with trypsin, enriched for phosphopeptides and subjected to untargeted data-dependent acquisition by LC-MS/MS. Data analysis was performed jointly for all samples, and intensity values of triplicates were averaged.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Solanum Peruvianum

TISSUE(S): Plant Cell, Cell Suspension Culture

DISEASE(S): Disease Free

SUBMITTER: Fatima Haj Ahmad

LAB HEAD: Andreas Klaus Schaller

PROVIDER: PXD010819 | Pride | 2019-06-17

REPOSITORIES: Pride

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			Action	DRS
	A17_00_1.raw	Raw
	A17_00_2.raw	Raw
	A17_00_3short.raw	Raw
	A17_02_1.raw	Raw
	A17_02_2.raw	Raw

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Publications

The Systemin Signaling Cascade As Derived from Time Course Analyses of the Systemin-responsive Phosphoproteome.

Haj Ahmad Fatima F Wu Xu Na XN Stintzi Annick A Schaller Andreas A Schulze Waltraud X WX

Molecular & cellular proteomics : MCP 20190528 8

Systemin is a small peptide with important functions in plant wound response signaling. Although the transcriptional responses of systemin action are well described, the signaling cascades involved in systemin perception and signal transduction at the protein level are poorly understood. Here we used a tomato cell suspension culture system to profile phosphoproteomic responses induced by systemin and its inactive Thr17Ala analog, allowing us to reconstruct a systemin-specific kinase/phosphatase ...[more]

PMID: 31138643

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