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Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome

ABSTRACT: In the presented datasets, we use newly developed isotopically labeled desthiobiotin azide (isoDTB) tags to residue-specifically quantify cysteines in a variety of bacterial strains and a human cell line with consistently high coverage. We e.g. quantify 59% of all cysteines in essential proteins in Staphylococcus aureus. We discover 88 cysteines with high reactivity, which correlates with functional importance. Furthermore, we identify 268 cysteines that are engaged by covalent ligands. Overall, a comprehensive map of the bacterial cysteinome is obtained.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Stephan M. Hacker

LAB HEAD: Dr. Stephan M. Hacker

PROVIDER: PXD015457 | Pride | 2019-12-02

REPOSITORIES: Pride

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			Action	DRS
	180326_SMH_180307_P17.raw	Raw
	180326_SMH_180307_P18.raw	Raw
	180326_SMH_180307_P19.raw	Raw
	180326_SMH_180307_P20.raw	Raw
	180326_SMH_180307_P21.raw	Raw

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