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Multiplexed quantitative proteomics of extracellular vesicles released by endMSCs (EV-endMSCs)

ABSTRACT: The extracellular vesicles released by endMSCs (EV-endMSCs)were characterized by multiplexedquantitative proteomics approach. We hypothesized that inflammatory priming of adult stem cells may contribute to increase their therapeutic effectiveness, thus, extracellular vesicles derived from IFN-γ-primed endMSCs (IFNγ/EV-endMSCs) were compared to control counterparts (n=3 patients).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Inmaculada Jorge

LAB HEAD: Javier G Casado

PROVIDER: PXD015465 | Pride | 2019-12-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	FR_ALL_Exos_JGC.msf	Msf
	FR_ALL_Exos_JGC.raw	Raw
	FR_ALL_bis_Exos_JGC2.msf	Msf
	FR_ALL_bis_Exos_JGC2.raw	Raw
	FR_Exos_JGC_Fr1.msf	Msf

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1 - 5 of 28

Publications

Unraveling the Molecular Signature of Extracellular Vesicles From Endometrial-Derived Mesenchymal Stem Cells: Potential Modulatory Effects and Therapeutic Applications.

Marinaro Federica F Gómez-Serrano María M Jorge Inmaculada I Silla-Castro Juan Carlos JC Vázquez Jesús J Sánchez-Margallo Francisco Miguel FM Blázquez Rebeca R López Esther E Álvarez Verónica V Casado Javier G JG

Frontiers in bioengineering and biotechnology 20191220

Endometrial-derived Mesenchymal Stem Cells (endMSCs) are involved in the regeneration and remodeling of human endometrium, being considered one of the most promising candidates for stem cell-based therapies. Their therapeutic effects have been found to be mediated by extracellular vesicles (EV-endMSCs) with pro-angiogenic, anti-apoptotic, and immunomodulatory effects. Based on that, the main goal of this study was to characterize the proteome and microRNAome of these EV-endMSCs by proteomics and ...[more]

PMID: 31921832

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Extracellular vesicles (EV) from IFNγ-primed cardiosphere-derived cells (CDCs)

Project description:Characterization of proteins in extracellular vesicles (EV) from cardiosphere-derived cells (CDCs) of a clinically relevant pig model. Additionally, considering that priming stem cells with inflammatory stimuli may increase the therapeutic potential of released vesicles, here we studied the dynamic changes that take place in the EV from IFNγ-primed CDCs.

2020-05-15 | PXD016434 | Pride

Exposure to aerosol of an e-vapor device with MESH™ technology had a lower biological impact than exposure to cigarette smoke on human bronchial and alveolar culture models: an in vitro systems toxicology assessment.

Project description:The harmful effects of cigarette smoke exposure on the respiratory tract are widely known. Exposure to aerosol from electronic vapor (e-vapor) products has been suggested to result in less risk of harm to smokers than CS exposure. Many studies have assessed the potential toxicity of the aerosol from e-vapor products in vitro. However, most studies have only tested the effects of liquid formulations applied directly to cell cultures but not those of the aerosolized formulations. In this study, we examined the effects of acute exposure on human organotypic bronchial epithelial culture and alveolar tri-culture models to an aerosol generated by an e-vapor device that uses the MESH™ technology (IQOS® MESH, Philip Morris International) and to CS from the 3R4F reference cigarette. In contrast to 3R4F CS exposure, exposure to the IQOS MESH Classic Tobacco aerosol did not cause cytotoxicity in either of the two lung culture models, despite resulting in greater concentrations of deposited nicotine. Ciliary beating frequency in bronchial cultures was not impacted in response to IQOS MESH aerosol exposure, whereas CS exposure caused a marked decrease in the frequency and the cilia beating active area. We complemented the histological and functional findings with quantitative analysis of the molecular changes in the exposed cultures. Global mRNA expression profiles and secreted protein profiles revealed a significantly lower impact of exposure to IQOS MESH aerosol than to 3R4F CS exposure. Overall, our study using whole aerosols for the exposure shows a much reduced impact of IQOS MESH aerosol in comparison to CS exposure in both bronchial and alveolar cultures, even at greater nicotine doses.

2021-01-31 | E-MTAB-10071 | biostudies-arrayexpress

Clinical exome sequencing of cells freshly isolated from 12 human colorectal carcinoma patients (tumor endothelial cells, normal colon endothelial cells, PBMCs, n=12 each) in comparison to microdisected tumor DNA (n=11) from corresponding FFPE-tissue blocks

Project description:Clinical exome sequencing of cells freshly isolated from 12 human colorectal carcinoma patients (tumor endothelial cells, normal colon endothelial cells, PBMCs, each n=12) in comparison to DNA isolated from microdissected tumor cells (n=11) from corresponding FFPE-tissue blocks

2023-05-01 | E-MTAB-10521 | biostudies-arrayexpress

Effects of chronic social stress on mitochondrial and mitochondrial-associated membrane protein networks in the mouse brain

Project description:Stressors challenge metabolic processes to adapt to ever-changing environmental conditions and herein, the brain is exceptionally amenable to change. Simultaneously, the high cerebral energy demands may render the brain particularly vulnerable to the metabolic consequences of psychological stress. Mitochondria are central organelles within energy metabolism, known to dynamically adjust function according to shifts in metabolic demands, and thus pivotal in the stress response. It is largely unclear how mitochondria respond to a psychological stressor and how mitochondria orchestrate their actions with other organelles, such as the endoplasmatic reticulum (ER). To address these questions we subjected male mice to chronic social defeat stress (CSD) and analyzed different subcellular brain fractions using label-free proteomics: mitochondrial associated membranes (MAMs), ER, and crude and pure mitochondrial fractions. Although insufficient to warrant a protein pathway analysis, we identified a few noteworthy differentially regulated proteins. These included decreased levels of SH3GL2 (SH3 Domain Containing GRB2 Like 2 or Endophilin 1) in the crude mitochondrial fraction of stressed mice, which may be related to impaired presynaptic calcium influx. In line, CACNA1A (Calcium Voltage-Gated Channel Subunit Alpha1 A) was absent in the pure mitochondrial fraction from CSD-exposed mice, a finding also suggestive of an impaired mitochondrial calcium flux. Then, in the MAM-fraction we observed an absence of HK3 (hexokinase 3) in CSD-treated mice, which may be linked to the CSD-induced cerebral hyperglycemia reported previously. Although verification is still required, our findings revealed a number of differentially expressed proteins possibly critical in the metabolic response to chronic stress.

2022-10-13 | PXD015983 | Pride

RNA-seq of NvNcol3::mOrange2 positive and NvNcol3::mOrange2 negative cells from Nematostella vectensis

Project description:NvNcol3::mOrange2 is a stable transgenic line that labels cnidocytes(stinging cells) of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvNcol3::mOrange2 positive and negative cells were enriched by FACS.

2021-07-01 | E-MTAB-9556 | biostudies-arrayexpress

Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development

Project description:Several in vitro models have been developed to recapitulate mouse embryogenesis solely from embryonic stem cells (ESCs). Despite mimicking many aspects of early development, they fail to capture the interactions between embryonic and extraembryonic tissues. To overcome this difficulty, we have developed a mouse ESC-based in vitro model that reconstitutes the pluripotent ESC lineage and the two extra-embryonic lineages of the post-implantation embryo by transcription factor-mediated induction. This unified model recapitulates developmental events from embryonic day 5.5 to 8.5, including gastrulation, and formation of the anterior-posterior axis, brain, a beating heart structure, and the development of extraembryonic tissues, including yolk sac and chorion. Comparing single-cell RNA sequencing from individual structures with time-matched natural embryos identified remarkably similar transcriptional programs across lineages, but also showed when and where the model diverges from the natural program. Our findings demonstrate an extraordinary plasticity of ESCs to self-organize and generate a whole embryo-like structure.

2022-09-17 | E-MTAB-12140 | biostudies-arrayexpress

RNA-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)

Project description:The purpose of this experiment was to compared the transcriptome of hepatocyte-like cells (HLCs) generated in vitro and adult primary human hepatocytes (PHHs). HLCs were differentiated from either hESCs or hIPSCs using previously established protocols (Hannah et al 2013; Segeritz et al 2018). Undifferentiated hIPSCs were used as control to confirm differentiation status. PHHs were commercially sourced as well as freshly isolated from donors.

2022-08-11 | E-MTAB-10634 | biostudies-arrayexpress

RNA-seq of NvElav1::mOrange positive and NvElav1::mOrange negative cells from Nematostella vectensis

Project description:NvElav1::mOrange is a stable transgenic line that labels a large fraction of the nervous system of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvElav1::mOrange positive cells were enriched by FACS.

2020-02-28 | E-MTAB-8794 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana wild type and asy1 Col-0 × Ws-4 F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 187 wild type and 169 asy1 Col-0 x Ws-4 F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev; Lawrence et al, 2019 Current Biology).

2020-05-18 | E-MTAB-8715 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana asy1/+ Col × Ler F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 191 asy1/+ Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev; Lawrence et al, 2019 Current Biology).

2020-05-18 | E-MTAB-8725 | biostudies-arrayexpress

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